通过对10头15kg体重试验猪实施门静脉血管插管等外科手术,并结合回肠肠系膜静脉灌注对氨基马尿酸(PAH)技术,探讨半乳甘露寡糖对生长猪门静脉血浆流率(PVPF)和血液流率(PVBF)、氨基酸和葡萄糖的净吸收量及耗氧量的影响。10头杜长大阉公猪随机分为2个处理.每个处理5头,单笼饲养于可调节不锈钢代谢笼内,经过14d适应期后,在门静脉、肠系膜静脉、颈动脉安装插管,开始15d正式试验。对照组饲喂玉米-豆粕型基础饲粮。试验组饲喂基础饲粮+0.20%半乳甘露寡糖。结果表明,经过15d饲喂期后。与对照组相比,饲粮添加半乳甘露寡糖可显著降低试猪食后8h内门静脉的平均血浆流率和血液流率。显著提高食后8h内门静脉对氨基酸和葡萄糖的净吸收量.显著降低食后8h内门静脉的耗氧量,也就是说半乳甘露寡糖可通过减少生长猪小肠黏膜对氨基酸和葡萄糖的氧化而增加肠外组织对其吸收。
The study was conducted to determine the effects of galacto-mannan-oligosaccharides (GMOS) on portal vein plasma flow (PVPF) rate, portal vein blood flow (PVBF) rate, net appearance (% of intake) of amino acids and glucose in portal blood, and O2 consumption by the portal vein-drained organs in growing pigs. After 14 days adapling periods in mental metabolile cage, Ion pigs (initial body weight of 15 kg) were fitted wilh chronic cathelers in carolid arlery, portal vein, and ileal mesenteric vein, simultaneously. Pigs were randomly allocated to two groups and fed corn-soybean meal-based diets supplemented with 0 or 0.20% GMOS (n=5 per group). At the end of 15 days feeding trial, blood and plasma flows were measured with infusion of paminohipuric acid (PAH) into the ileal vein. At 8 h after feeding, dietary supplementation with GMOS decreased (P〈0.05) PVPF and PVBF in portal vein, increased (P〈0.05) net absorption of amino acids and glucose into portal vein, and decreased (P〈0.05) O2 consumption by the portal vein-drained organs, when compared with the control pigs. Our results suggest that GMOS reduces the oxidation of amino acids and glucose by the small-intestinal mucosa in growing pigs, thereby enhancing their availabilities for utilization by extraintestinal tissues and cells.