2009年,在桃江和浏阳采集了26个品种上的稻瘟病标样,经单孢分离获得162个稻瘟病菌株,利用10对SSR引物进行了遗传多样性分析。结果表明,在0.80的相似水平上,162个菌株划分为23个宗谱,L01为优势宗谱,占总菌数的72.8%;在2个病圃相同品种上分离得到的59个菌株归属16个宗谱,桃江病圃的有13个宗谱,浏阳病圃的有5个宗谱,其中相同的宗谱有2个,相异的宗谱有14个,各占12.5%和87.5%;空中捕捉的21个菌株归属6个宗谱,属优势宗谱L01的有16个菌株,占76.2%,说明空中捕捉的菌株多数来自于病圃中的优势宗谱。研究还表明,2个病圃中的稻瘟病菌均具有遗传多样性,且特点各异;病菌宗谱与水稻品种之间有一定的相关性;来源于同一病圃的菌株亲缘关系较为密切。
162 isolates of Magnaporthe oryzae,collected from 26 varieties in the rice blast nurseries in Taojiang and Liuyang in 2009,were analyzed for genetic diversity by using 10 pairs of SSR markers.The results showed that at 0.80 similar level,162 isolates were classified into 23 genetic lineages,and L01 was the dominant lineage which account for 72.8% of the total isolates;59 isolates,from the same cultivars planted in the two rice blast nurseries,were classified into 16 genetic lineages,in which Taojiang nursery had 13 genetic lineages and Liuyang nursery had five genetic lineages,respectively,and the same lineages and different lineages among them were 2 and 14,respectively,which account for 12.5% and 87.5%,respectively;the 21 isolates trapped from the air were grouped into six genetic lineages,in which 16 isolates belonged to predominant lineage L01,which account for 76.2%,so it indicated that the trapped isolates from air mostly came from the predominant lineage.The results also indicated that Magnaporthe oryzae in both rice blast nurseries all had the genetic diversity and characteristics were different;there was a certain correlation between the genetic lineages and the rice varieties,and the isolates from the same rice blast nursery had closer genetic relationship.