中文摘要：

目的观察并初步分析1-[4-amino-2-（ethoxymethyl）imidazo[4，5-c]quinolin-1-yl]2-methylpropan-2-ol（R848）协同CD3抗体诱导C57BL/6小鼠脾脏中淋巴细胞发生的功能改变。方法分离正常C57BL/6小鼠脾脏的淋巴细胞，分别在anti-CD3、R848及anti-CD3＋R848的作用下培养72h。通过MTT法观察淋巴细胞的增殖情况：采用ELISA的方法检测脾脏淋巴细胞的IFN-γ和IL-4的产生情况；使用细胞内细胞因子染色的方法检测CD4＋T和CD8＋T细胞的IFN-γ和IL-4的产生情况；再用细胞表面分子染色的方法检测R848诱导不同淋巴细胞群活化的情况。结果单独使用R848对淋巴细胞的增殖作用不明显。但anti-CD3＋R848可促进淋巴细胞的增殖；R848能辅助anti-CD3明显诱导脾CD4＋T和CD8＋T细胞分泌IFN-γ和IL-4；R848能够使淋巴细胞活化，且以B细胞亚群为主。结论R848能够协助CD3抗体促进脾脏淋巴细胞的增殖，分泌IFN-γ和IL-4，其机制与R848能明显促进B淋巴细胞活化有关。

英文摘要：

This study performed to detect and analysis the roles of R848 on synergy with anti-CD3 in inducing spleen lymphocytes functional changes in C57BI/6 mice. Lymphocytes were isolated from spleens of normal C57BL/6 mice and cultured with medium, anti-CD3, R848 or anti-CD3 ＋R848 for 72 h. Then, MTT＋ experiment was adopted to analyze the proliferation of lymphocytes; the level of IFN-γ and IL-4 in the supernatants of cultured cells was measured by ELISA; the content of different lymph cell populations and intracellular cytokines （IFN-γ and IL-4） were detected by FACS. Results indicated that effects of R848 on lymphocyte proliferation were not obvious, but anti-CD3 plus R848 could promote anti-CD3 to induce lymphocyte proliferation. Also, R848 and anti-CD3 had synergistic effect on the production of IFN-γ and IL-4 by CD4＋T and CD8＋T cells. Furthermore, R848 could induce lymphocyte activation, especially B cells. In conclusion, R848 could assist the effects of anti- CD3 of inducing spleen lymphocyte proliferation and IFN-γ and IL-4 secreting, and the mechanism might related to R848-induced B lymphocyte activation.