以猪为材料利用细胞计数仪法研究了骨髓基质干细胞的渗透特性,包括细胞的等渗体积、低渗或高渗溶液中细胞的平衡体积及细胞的不可渗体积.结果表明,在等渗条件下,骨髓基质干细胞的平均体积为5248.4μm~3,相当直径d为21.6μm;细胞体积随溶液渗透压的变化规律符合Boyle van’t Hoff关系式,据此得到猪骨髓基质干细胞的不可渗体积V_b=0.36Vi,为1902.5μm~3.
The osmotic characteristics of pig bone marrow stromal stem cells are exper- imental measured using electronic sizing technique.The cell volume measured is 5248.4μm~3, the relative diameter 21.6μm,at isotonic osmolality.The cell volume change is measured after the cells expose and equilibrate to different anisosmotic conditions.The cell volume is found to be a linear function of the reciprocal of the extracellular osmolality(Boyle van t Hoff plot).The non-osmotic volume of the cell is 36% of the cells isoto...