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中文摘要：
探讨载入海藻糖对红细胞冷冻干燥保存的效果．利用硫酸蒽酮比色法测量细胞内海藻糖的含量，对载入海藻糖的红细胞进行了冷冻干燥保存．结果表明，细胞载入海藻糖的最高浓度为35mmol／L，经冷冻干燥后的细胞回收率与血红蛋白回收率提高非常明显，分别为冷冻干燥前的（59．78±4．51）％和（57．49±3．69）％；渗透脆性与新鲜红细胞无显著差异．载入海藻糖对红细胞的冷冻干燥保存效果有密切作用．
英文摘要：
Effects of intracellular trehalose on red blood cells by freeze drying are investigated. Trehalose is loaded into fresh red blood cells after 7 hours＇ incubation at 37℃ in various concentration trehalose solutions （400,600,800,1 000 mmol/L）. The results indicate that the intracellular trehalose concentration is increased with the increasing concentration of extracellular trehalose solution. The maximum concentration of intracellular trehalose is 35 mmol/L in this study. Red blood cells loaded with trehalose are lyophilized. Results show that the viability （59.78 ± 4.51） % and the recovery of hemoglobin （57.49 ± 3.69） % of incubated group increase remarkably compared to those of control group. Osmotic fragility data show that trehalose exerts osmotic protection on RBCs during freezedrying.
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