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中文摘要：
以二甲基亚砜（Me2SO）为低温保护剂,在就Me2SO浓度、处理温度及时间对真皮成纤维细胞的活性影响进行研究的基础上,研究了Me2SO浓度对低温保存后组织工程化真皮活性的影响.采用胎盼蓝拒染法和四唑盐（MTT）比色法检测细胞存活率.结果表明,Me2SO浓度、预处理时间及温度对细胞及组织的活性有明显影响.将添加低温保护剂后的细胞悬液在4℃下保持20 min有利于保持较高的细胞活性;且当Me2SO的体积分数在10%-20%时,可使低温保存后的组织工程化真皮组织的细胞活性保持在60%以上.
英文摘要：
In order to optimize the cryopreservation protocols of tissue engineered dermal equivalent, the effects of cryoprotectant concentration and the loading conditions on the cell viability of tissue-engineered dermal replacement were studied with dimethyl sulfoxide（Me2SO） as the cryoprotectant. The effects of Me2SO concentration and the exposure time/temperature to Me2SO on the cell viability of dermal fibroblasts were studied. Then experiment was conducted to investigate the effect of Me2SO concentration on the cell viability of freeze-thawed tissue-engineered dermal replacement, under the selected pretreatment conditions. Viabilities of the fibroblast and the dermal slices were determined by using the Trypan Blue staining and the modified MTT assay, respectively. The results indicate that the cryoprotectant concentration （Me2SO） and the loading conditions （pretreatment time and tempera- ture） have significant effects on the cell viability of the dermal fibroblast and the tissue-engineered dermal equivalent. In order to maintain a relatively higher cell viability（≥60 % ） during： the cryoprotectant loading stage, a combination of 4 ℃ and 20 min is preferable. And if the Me2SO concentration ranged from 10% to 20% ,the freeze-thawed dermal equivalent can retain the cell viability over 60%.
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