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中文摘要：
本研究对分离自二点委夜蛾Athetis lepigone（M？schler）越冬幼虫体内的微孢子虫进行形态学和分子生物学鉴定,初步证明该微孢子虫与家蚕微孢子虫具有最近的近缘关系,属于微孢子纲,微孢子虫目,微粒子科,微粒子属Nosema;测定了该微孢子虫不同浓度对不同龄期二点委夜蛾的致病力,在接种1×107孢子/m L浓度时,对初孵、2龄、3龄幼虫8 d的校正死亡率分别达到91.6%、83.35%和70.4%,显示出对靶标害虫具有较好的毒力;对田间自然条件下微孢子虫在二点委夜蛾种群中的流行动态进行了调查评估,结果表明微孢子虫在二点委夜蛾种群数量的控制中起到一定作用,具有潜在的应用价值。
英文摘要：
A microsporidian was isolated from Athetis lepigone overwintering larvae, based on morphological and molecular biology experiments, it was preliminarily proved that the rnicrosporidian had the closest relationship with Nosema bombycis, which belong to Microsporea, Microspoida, Nosematida, Nosema. Bioassay tests showed that microsporidian had high toxicity against different instars larvae of A. lepigone. The corrected mortality of the concentration I × 107 spores/mL to initial hatching larva, 2nd instar larva and 3rd instar larva at 8 d after infection were 91.6%, 83.35% and 70.4%. In field investigation, we concluded that the microsporidian could controll A. lepigone populations with potential application value.
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