中文摘要：

目的：观察佐剂关节炎（Adjuvant arthritis,AA）大鼠肺功能、外周血调节性T细胞（Treg）及肺组织Notch通路变化。方法：将20只SD大鼠随机分为正常对照组（NC）和模型对照组（MC）,每组10只;向MC组大鼠右后足跖皮内注射弗氏完全佐剂0.1 ml致炎,复制成AA模型;致炎18天后,观察两组大鼠足跖肿胀度（E）、关节炎指数（AI）、肺功能、肺组织形态学变化,采用流式细胞术检测外周血Treg,PT-PCR法检测肺组织Notch受体及配体表达。结果：AA大鼠E、AI、1秒内平均呼气流量（FEV1/FVC%）、肺组织Notch3、Notch4及Delta1的表达明显升高;75%肺活量的最大呼气流量（FEF75）、用力最大呼气流量（PEF）、肺动态顺应性（Cldyn）降低,外周血CD4＋CD25＋Treg、肺组织Notch1、Jagged1、Jagged2的表达水平显著降低（P〈0.01或P〈0.05）。相关分析显示,肺功能参数FVC、FEF75与CD4＋CD25＋Treg呈正相关,FEF50、MMF与Jagged1、Notch1呈正相关;FEF25、FEF50、PEF与Delta1、Notch3、Notch4呈负相关（P〈0.01或P〈0.05）。结论：AA大鼠发生关节炎症同时,出现肺功能、Treg降低及Notch通路的变化;肺功能与Treg、Notch受体/配体呈高度相关性,提示Treg和Notch通路可能参与肺功能降低的过程。

英文摘要：

Objective：To observe the changes of lung function,regulatory T cells（Treg） of peripheral blood,Notch pathway of lung tissueby RT-PCR in adjuvant arthritis（AA） rats.Methods：20 SD rats were randomly divided into normal control（NC） group and model control group（MC）,10 in each.The MC group were intracutaneously injected with 0.1ml of Freund＇s complete adjuvant in the right hindlimb.To observe the changes of rat paw swelling（E）,arthritis index（AI）,lung function,lung tissue morphology,Treg in peripheral blood,Notch pathway in lung tissue by RT-PCR,after proinflammatory 18d.Results：The expression of E,AI,1 second average expiratory flow（FEV1/FVC %）,Notch3,Notch4,and Delta1 in lung tissue were significantly increased in AA rats.The expression of 75% of vital capacity,maximum expiratory flow（FEF75）,forced peak expiratory flow（PEF）,reduced pulmonary dynamic compliance（Cldyn）,CD4＋CD25＋Tregs,Notch1,Jagged1,Jagged2 were significantly lower（P 0.01 or P 0.05）.Correlation analysis show that FVC,FEF75 were positively correlated with CD4 ＋CD25 ＋Treg.FEF50,MMF were positively correlated with Jagged1,Notch1.FEF25,FEF50,PEF were negatively correlated with Delta1,of Notch3,Notch4（P 0.01 or P 0.05）.Conclusion：It was occurred arthritis in AA rats,the level of lung function,Treg were reduced,and the changes of Notch pathway.The Correlations between Lung Function and CD4 ＋ CD25 ＋ Treg,Notch Receptor/Ligand suggesting that Treg and the Notch pathway may be involved in lung function reduction process.