中文摘要：

种子活力形成于种子发育的脱水阶段,与以往理解不同,近来研究表明种子脱水不仅仅是一个简单的失水过程,脱水与萌发存在一些相同的基因表达和代谢特征。萌发始于吸水膨胀,种子代谢恢复,胚根突破胚乳和种皮等外围包被组织完成萌发。种子萌发的质量关键在于储藏的mRNA的质量,另外,蛋白质稳定性和DNA完整性影响萌发的表型。激素作为一种信号小分子物质,浓度极小甚至是趋于零时对种子萌发仍具有非常重要的作用,近年来研究表明ABA/GAs的阈值范围调控种子休眠与萌发,其中,ABA几乎作用于种子萌发的全部过程,GA的作用并不像ABA那样广泛,主要在胚根突出时发生作用,且ABA和GA彼此抑制对方的合成与分解代谢基因,它们均可调控α-淀粉酶基因的转录。除ABA和GA外,近来发现AUX参与调控种子的休眠与萌发,其对胚根突出的调控比对子叶开展更加精细,AUX信号与ABA信号存在交叉,AUX通过其响应蛋白ARF10/16间接调控ABA信号通路中ABI3的稳定性来调控拟南芥种子休眠与萌发。与光照条件下相比,在土壤中萌发的幼苗将形成一个特异性的组织＂顶钩＂,它的主要作用是在幼苗＂顶土＂时保护顶端分生组织,＂顶钩＂形成与生长素的不均匀分布有关。为了提高种子活力,引发技术被运用于生产,引发的关键在于控制种子＂萌而未发＂,在＂回干窗口＂内及时脱水,引发过程中种子储藏的mRNAs和蛋白已经执行功能,回干后这种分子机制被＂牢记＂,再吸胀的种子可以迅速整齐的萌发。除毒害分子外,ROS还作为信号分子参与调控种子休眠释放、胚乳松弛和贮藏物动员,且其与激素分子ABA和GA等存在交互作用,ROS还参与蛋白的翻译和翻译后修饰调控种子萌发吸水和贮藏物动员。在种子萌发过程中,甲硫氨酸代谢是代谢核心,其代谢产物广泛参与调控种子萌发的一些生理生化反应,如：D

英文摘要：

Seed acquired vigor during desiccation stage in seed development,different from the previous opinions,new evidence insisted that seed desiccation is not only involved in water loss process,but also as a significant proportion of the gene expression and metabolic signatures of which resemble those characterized seed germination,implied that the preparation of the seeds for germination began already during seed desiccation.The germination of seeds initiated from water-uptake,accompanied by metabolic recovery,then radicle breaked through endosperm and seed coat and other peripheral completed germination.The main contributor of seed germination success is the quality of the messenger RNAs stored during embryo maturation in the mother plant.In addition,proteostasis and DNA integrity play a major role in the germination phenotype.Plant hormone,as a signal,concentration extremely fewer even approximate to zero,was also important for seed dormancy release and germination.Recently,more and more ideas have considened that the ABA/GA ratio regulats the metabolic transition required for germination.GAs,although required for the completion of germination,are not directly involved in many processes taking place during germination like ABA,which occurred at a stage coinciding with or very close to radicle emergence.It appears that reciprocal downregulation of the respective metabolic pathways accounts for a significant part of GA and ABA interplay,and the α-amylase gene,is transcriptionally regulated by both ABA and GA.In addition to the ABA and GA,recently research found IAA finely regulated radicle emerge that more strictly than cotyledons open during seed germination.Auxin action in seed dormancy and germination requires the auxin response factors 10 and 16 to indirectly control the expression of ABI3.Compared with germination in light condition,seedlings germinated in soil will form a specific organization ‘Apical hook＇,its main role was to protect the ‘SAM＇.Auxin,accumulating at the concave side,was critical for t