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中文摘要：
目的比较天山雪莲细胞培养物与原药材中紫丁香苷含量的差异。方法建立了一种HPLC-DAD方法测定紫丁香苷的含量：色谱柱采用Agilent Zorbax SB—C18柱（4．6mm×250mm，5μm），柱温为25℃，流动相为乙腈-水（10：90），流速1．0mL·min^-1，检测波长220nm。结果与天山雪莲原药材相比，细胞培养物中紫丁香苷的含量在液体培养和固体培养方式下，分别提高了20．96和55．50倍。结论利用细胞培养方式生产紫丁香苷具有良好的开发前景。
英文摘要：
OBJECTIVE To compare the syringin content in cell cultures with that in botanical drug of Saussurea involucrata. METHODS A HPLC-DAD method was developed as follows： chromatographic separation was achieved on an Agilent Zorbax SB - C18 column （4. 6 mm ×250 mm,5 μm） at 25 ℃ and the mobile phase was a mixture of acetonitrile-water （10：90） detected at 220 nm at a flow rate of 1.0 mL · min^-1. RESULTS Compared with the botanical drug, the content of syringin in cells was improved 20. 96 times by liquid culture and 55. 50 times by solid culture. CONCLUSION It has a good prospect to acquire syringin by plant cell culture.
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