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一种寄生家蚕被毛孢的鉴定及产孢条件优化
  • ISSN号：0253-2654
  • 期刊名称：《微生物学通报》
  • 时间：0
  • 分类：S763.757.2[农业科学—森林保护学;农业科学—林学]
  • 作者机构：[1]贵州大学真菌资源研究所,贵州贵阳550025
  • 相关基金：国家自然科学基金项目（No.30360031）;国家自然科学基金重大子项目（No.31093440）;贵州省科技基金项目（No.黔科合J字[2011]2160号）;贵州大学引进人才项目（No.2011-10）
作者： 瞿娇娇[1], 邹晓[1], 周叶鸣, 韩燕峰[1], 梁宗琦[1]
关键词： 被毛孢属, 鹿儿岛被毛孢, 家蚕, 产孢条件, Hirsutella Pat., Hirsutella satumaensis, Bombyx mori Linnaeus., Sporulation
中文摘要：

【目的】对寄生家蚕的真菌被毛孢进行鉴定和孢子培养进行研究。【方法】采用形态特征比较和内转录间隔区（ITS）序列构建系统树进行鉴定，通过单因素筛选和正交试验进行产孢条件优化。【结果】根据形态特征比较和系统发育分析，该真菌为鹿儿岛被毛孢Hirsutella satumaensis Aoki。较优产孢条件为（质量体积比）：蛋白胨3%，葡萄糖1%，蚕蛹粉1.5%，维生素B1 1%，硫酸镁0.05%，磷酸二氢钾0.1%，琼脂2%，蒸馏水1 000 ml，25 ℃。【结论】鹿儿岛被毛孢为已知种，文中对其显微特征进行了重新描述并补充相关分子系统学资料；产孢条件的优化可为该类群真菌孢子的获得与应用提供参考。

英文摘要：

[Objective] The objectives are to identify a parasitic Hirsutella Pat. of Bombyx mori Linnaeus. and to research the spore culture of this fungus. [Methods] The comparison of morphological characteristics and internal transcribed spacer （ITS） sequence analysis were used for identification of specimens. single factor test and orthogonal test were carried out for studying sporulation conditions optimization. [Results] According to the morphological characterization and phylogenetic tree, the fungus was identified as Hirsutella satumaensis Aoki. The optimum conditions for sporulation is： peptone 3%, glucose 1%, silkworm chrysalis powder 1.5%, vitamin B1 1%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, agar 2%, distilled water 1 000 ml, 25 ℃. [Conclusion] H. satumaensis Aoki, a known species in China, which microscopic characteristics were described again and the associated molecular systematics data were added in this paper. The sporulation conditions optimization could provide a reference for the spores acquisition and application of this group of fungi.

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期刊信息
  • 《微生物学通报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院微生物研究所 中国微生物学会
  • 主编：赫荣乔
  • 地址：北京市朝阳区北辰西路一号院3号中国科学院微生物研究所内B401室
  • 邮编：100101
  • 邮箱：xuj@sun.im.ac.cn
  • 电话：010-64807511
  • 国际标准刊号：ISSN：0253-2654
  • 国内统一刊号：ISSN：11-1996/Q
  • 邮发代号:2-817
  • 获奖情况:
  • 1992年中国科学技术协会优秀学术期刊三等奖,1992年国家科委中共中央宣传部新闻出版署“全国优...,2000年中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）,中国北大核心期刊（2000版）
  • 被引量:29487