中文摘要：

在一次多菌株的生物活性测定中，发现一株被毛孢菌株RCEF0851菌丝体提取物具有较强的抗肿瘤CHO细胞的活性。本研究用不同溶剂对活性组分进行了提取试验，发现乙酸乙酯能最大程度地提取出该活性成分。分离试验表明该提取物较适于用反相柱SynergiHydro进行分离。活性指导下的分离得到一活性组分的纯品Hh-1。该组分在40gg／mL时对CHO细胞的抑制率达到83．61％。高分辨质谱分析表明该活性化合物的可能分子式是C30H22O10。该分子为首次从虫生真菌中发现。

英文摘要：

During a screening of fungal strains with anti-tumor activity against CHO cells, a strain named RCEF0851 of Hirsutella sp. whose extract exhibited significant bioactivity was found. Extraction＇ tests with different solvents revealed that acetylacelate was the best solvent for obtaining the extraction with active components. The extract can be best purified by using RP column Synergi Hydro. Bioactive guided isolations resulted in obtaining a pure active compound Hh-1. The inhibition rate of the compound against CHO cells reached 83.61% at the concentration of 40μg/mU HPLC-HRMS analysis revealed that the possible molecular formula of the active compound was C30H22O10 which was isolated from entomogenous fungi for the first time.