以已知核苷为标样,并以冬虫夏草生药作对比,利用反相高效液相色谱法对细脚拟青霉RCEF0441深层发酵菌丝体中核苷类化学成分进行分析。结果表明,细脚拟青霉RCEF0441菌丝体中主要含有胞苷、尿苷、鸟苷、肌苷、胸苷和腺苷6种核苷,其中胞苷的含量为冬虫夏草生药的3倍左右,肌苷和鸟苷的含量则分别是冬虫夏草生药的7和9倍左右,尿苷和腺苷的含量也显著高于对照品冬虫夏草生药(p<0.05)。
The nucleoside compositions in Cordyceps sinensis and fermentation mycelia of Paecilomyces tenuipes RCEF0441 were determined by high performance liquid chromatography (HPLC). The results showed that the nucleosides in mycelia of Paecilomyces tenuipes RCEF0441 include mainly adenosine, uridine, guanosine, inosine, cytidine and thymidine. The contents of cytidine, inosine and guanosine are about 3, 7 and 9 times of those in Cordyceps sinensis, respectively. Moreover, the contents of adenosine and uridine are ...