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中文摘要：
目的：探讨瑞典家系β-淀粉样前体蛋白（APPSWE）转基因小鼠发育过程中海马CA1区神经细胞凋亡规律。方法：取不同发育时间（P0、P7、P14、P30、P90、P180）APPSWE转基因模型鼠与同时间点对照鼠，Nissl染色观察海马结构和锥体细胞形态，免疫组织化学方法观察海马细胞内Caspase-3表达变化，RT-PCR检测Caspase-3mRNA表达变化。结果：随着小鼠的生长发育，P14时间点以后，模型组CA1区神经元Caspase-3阳性细胞密度比对照组高；RT-PCR检测结果与Caspase-3免疫组织化学结果基本一致。结论：APPSWE转基因小鼠发育中的海马神经细胞过度凋亡可能与阿尔茨海默病的发生、发展具有联系。
英文摘要：
Objective： To observe the rule of neuroapoptosis in developing hippocampus of transgenic mice with Alzheimer disease （APPSWE）. Methods： Wild type and APPSWE transgenic mice from postnatal day 1 to postnatal day 180 were included. Nissl staining, Caspase-3 immunohistochemistry and RT-PCR were used for the study. Results： From P14, the density of positive cells in CA1 area of AD model was obviously higher compared with that of age matched controls; the results of Caspase-3 immunohistochemistry were coincident with the data from RT PCR. Conclusion： The production and development of Alzheirner disease may be relevant to the neuroapoptosis of developing hippocampus.
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