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中文摘要：
目的观察小鼠Cajal—Retzius（cR）细胞的发生以及多种细胞周期相关蛋白的表达情况。方法各日龄共计83只小鼠，应用免疫荧光法、溴脱氧尿嘧啶核苷（BrdU）等技术对胚胎和出生后小鼠进行实验。结果1．CR细胞在E10和E11发生，它们主要分布在新皮质分子层和海马分子层，其密度随日龄的增长而逐渐降低。2．从E15到成年，细胞周期蛋白（cyclin）A2、cyclinE1和CDTl在CR细胞中持续表达，但始终未发现cyclinDI阳性的CR细胞。结论CR细胞对大脑发育有重要作用。CR细胞密度的减小趋势与新皮质发育的活跃程度相关。CR细胞已经退出了细胞周期而进入了G6期。如果CR细胞再次分裂，它们会因其轴突无法吸收并反向运输神经营养因子而凋亡。
英文摘要：
Objective To study Cajal-Retzius （CR） cells＇ histogenesis and their expressions of cell cycle-related proteins in mouse. Methods Total 83 mice were used. Immunofluorescent labeling and BrdU assay were carried out at various embryonic and postnatal ages. Results 1. The birthday of CR cells was at about embryonic day 10 （E10） or 11 （ E11 ）. CR cells mainly distributed in the molecular layer of neocortex and hippocampus. Their number decreased with age increasing. 2. From El5 to adult, Cyclin A2, Cyclin E1, and CDT1 were expressed continuously in CR cells, but there was no any expression of Cyclin D1 in CR ceils. Conclusion CR cells are important in the brain development. The decrease of CR cells with development is correlative with the activity of the neocortical development. CR cells in neocortex have been probably exited out cell cycle and steped in the Go phase. If the CR cells split again, they will apoptosis because of their axons can not absorb and reverse transport the neurotrophic factors.
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