中文摘要：

采用不同浓度酒精处理PC12细胞,观察酒精诱导细胞凋亡作用及其与线粒体凋亡途径的关系.结果显示,采用50、100、200、400和800mmol/L浓度的酒精处理去血清培养的PC12细胞24h后,细胞存活率分别是单纯去血清培养细胞存活率的85.66%、74.28%、62.47%、54.14%和50.35%,呈浓度依赖性,与对照组比较差异显著（P〈0.05）.Hoechst 33342/PI染色法观察到典型凋亡现象,如核型固缩、染色质凝集等.DNA琼脂糖凝胶电泳可见100～400mmol/L浓度酒精处理组呈现凋亡细胞典型梯状DNA.酒精能使PC12细胞线粒体膜电位下降并呈浓度依赖性.酒精致PC12细胞凋亡过程中,凋亡关键蛋白Caspase-3、Caspase-9表达量升高.上述结果表明,酒精能够诱导PC12细胞凋亡,诱导作用随浓度升高而增强且酒精诱导PC12细胞凋亡与线粒体凋亡途径有关.

英文摘要：

In this study,the author established the ethanol-induced apoptotic model of pheochromocytoma（PC12）cells.Using the model,the author examined the inhibition effect of ethanol on proliferation of PC12 cells by MTT assay.Ethanol at the concentrations of 50,100,200,400 and 800mmol/L decreased cell proliferation of PC12 cells to 85.66%,74.28%,62.47%,54.14% and 50.35%,respectively,compared to the control in serum-free media after 24 h,indicating a dose-dependent inhibitory effect of ethanol on cell proliferation（P〈 0.05）.The nucleimorphological changes were detected by using Hoechst 33342 staining and fluorescent microscopy.Addition of ethanol with different concentrations to PC12 cells resulted in a number of morphological changes that are characteristic of apoptosis,including cell shrinkage,chromatin compaction and nuclear fragmentation.Agarose gel electrophoresis of the DNA isolated from ethanol（100～400 mmol/L）treated cells maintained in the absence of serum yielded the classical DNA laddering pattern of 180 to 200 bp fragments suggestive of apoptosis and a concomitant dissipation of the mitochondrial membrane potential.In addition,alcohol increased the protein expression of caspase-9and caspase-3.These results indicate that alcohol induced apoptosis of PC12 cells in a dosedependent manner,and mitochondrial-dependent pathways were involved in alcohol induction of apoptosis of PC12 cells.