欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的探讨小鼠小脑皮质发育中神经元凋亡的规律和机制。方法用激活型Caspase-3多抗免疫组织化学标记及Hoechst 33258染色液染色,检测从出生至成年小鼠小脑皮质中神经元的凋亡,用Western blotting方法对小脑组织中Caspase-3和Caspase-8的活化片段进行半定量测定。结果外颗粒层、普肯耶细胞层和内颗粒层凋亡细胞密度最高分别在出生后第8d（P8）、P5及P9,P20各层凋亡细胞密度都很低。Caspase-3活化片段的表达量在P5较高,P5以后逐渐降低,至P14消失;Caspase-8活化片段的表达量从P0到P10都较高,P10以后逐渐降低,至P30消失。结论P0至P14是小脑皮质神经元凋亡的重要时期,通过启动Caspase-8的活化进而激活效应性Caspase-3的细胞凋亡途径存在于小脑皮质的塑型成熟过程。
英文摘要：
Objective To investigate the rules and mechanism of neuroapoptosis during the development in mouse cerebellar cortex. Methods The neuroapoptosis of cerebellar cortex was visualized by the active Caspase-3 pAb immunohistochemistry and Hoechst 33258 staining from postnatal day 0 （PO） to adult mice. Western-blotting was used to study the expression of Caspase-3 and Caspase-8 in the cerebellum. Results Apoptotic cells density was the highest at P8,175 and P9 in the external granular layer （EGL）,Purkinje cell layer （PCL） and internal granular layer （IGL） respectively, and the density of apoptotic cells of each layer was lower in cerebellar cortex at P20. Activated Caspase-3 was expressed higher at PS, then decreased gradually until the disappearance at P14, at the same time activated Caspase-8 was expressed higher from P0 to P10, and then disappeared at P30. Conclusion It was an important period for neuroapoptosis in cerebellar cortex from P0 to P14, and cell apoptosis pathway that Caspase-3 was activated through cleaved Caspase-8 exists during the molding and growth in. the mouse cerebellar cortex.
同期刊论文项目
同项目期刊论文
期刊信息
位置：