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中文摘要：
目的 对细胞膜红色荧光探针（DiI）标记的神经元进行光学转换,使其结果更稳定,更适于长期保存,并且经过处理可用于电镜观察. 方法 选用C57/B6J小鼠20只,采用DiI散射法标记视皮质神经元,对其进行光学转换后,制备电镜样本和观察超微结构. 结果 经过光学转换的DiI散射神经元可以更好地显示中枢神经系统内神经元的细微结构,其中包括树突小棘. 结论 经过DiI散射标记的细胞可以进行光学转换,转换后能清晰地观察到细胞的细微结构和超微结构,细胞器保存完好,突触结构清晰可见.
英文摘要：
Objective In order to make the labeled neurons and glia with 1,1＇- dioctadecyl-3,3,3＇, 3,- tetramethylindocarbocyanine perchlorate （DiI） diolistic labeling for long time preservation and ultrastructural study. Methods DiI diotistic assays were done on the sections of visual cortex of 20 C57/B6J mice to label neurons and glia, then the targeted neurons were photoeonverted and prepared for uhrathin section. Results The neurons after the photoconversion could still show fine shape with spines on dendrites and decent synapse ultrastructure. Conclusion After DiI diotistic labeling and photoconversation, the labeled neurons can present satisfied morphological details with either light microscopy or electron microscopy. The organelle is well and the synaptic structure is clear either.
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