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中文摘要：
目的探讨Cajal-Retzius（CR）细胞与海马锥体细胞顶树突发育以及海马片层化形成的关系。方法利用DiI示踪法标记槽通路和穿通通路，并采用微管蛋白（tubulin）、神经元核抗原（NeuN）和Reelin免疫荧光染色法，对120例小鼠苔藓纤维的发生、锥体细胞顶树突的生长以及海马分子层Reelin阳性的CR细胞分布进行观察。结果槽通路和穿通通路由内嗅皮质发出，分布于槽区和海马分子层，在槽区和海马本部分子层存在大量Reelin阳性细胞，提示Reelin阳性的神经细胞可能参与神经纤维的寻径。随着小鼠的发育，海马锥体细胞顶树突逐步生长，并向分子层伸入，构成海马内分子层；内分子层逐渐增宽，占据了光辉层和放射层。而外分子层（腔隙分子层）内含丰富的穿通纤维，其位置与Reelin阳性的CR细胞所在部位极为一致，提示两者有密切关系。结论Reelin阳性的CR细胞对槽通路、穿通通路的神经纤维寻径起着指导作用。此外，CR细胞的分布和发育特点提示，CR细胞对锥体细胞顶树突发育、海马片层化和区域化的形成起重要作用。
英文摘要：
Objective In order to understand how Cajal-Retzius cells are involved in the regulation to the apical dendrite growth of pyramidal cell and hippoeampal lamination. Methods With DiI tracing, the alvear path and perforaut path were visualized in 120 mice at various ages. The apical dendrite growth of pyramidal cells, the histogenesis of mossy fibers and the distribution of Reelin positive cells were studied with tubulin, neuronal nuclei and reelin immunofluorescent labeling. Results Alvear path and perforant path were started from entorhinal cortex, and they distributed in atveus and molecular layer of hippocampus. There were numerous Reelin positive cells in the alvear area and hippocampal molecular layer, suggesting those reelin positive cells were involved in the path-finding. With age increasing, the apical dendrites of pyramidal cell grew and extended into molecular layer to form the inner molecular layer. Later, the inner molecular layer widened gradually, and it occupied stratum lucidum and stratum radiatum. The perforant fibers in stratum lucunosum- moleculare were so coincident with the location of CR cells in CA1, suggesting regulation of CR cells in the apical dendrite growth in inner molecular layer. Conclusion Reelin positive cells can function as a guidance to the path-finding of alvear path and perforant path. Meanwhile, the developmental characteristics of CR cells suggest that CR cells play important roles in the dendritic growth of pyramidal cells, hippocampal lamination and hippocampal localization.
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