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中文摘要：
探讨重离子辐照对人舌鳞癌Tb细胞的凋亡及Bax／Bcl-2蛋白表达的影响。采用0、0．5、1．0、2．0、4．0Gy重离子束辐照人舌鳞癌Tb细胞，应用MTT法检测细胞存活，流式细胞技术检测细胞周期变化，Hoechst33258／PI复染法观察Tb细胞凋亡形态，并采用Western-blot法检测Bax／Bcl-2蛋白表达情况。结果发现，Tb细胞经^12C^6＋离子束辐照后存活率显著下降，呈剂量依赖性的生长抑制；Tb细胞呈现蓝色荧光浓集成团的凋亡形态，且凋亡比例随辐照剂量增加；G2/M期细胞百分数随照射剂量增加而增加（P〈0．05）。Western-blot结果显示Bax蛋白表达水平随辐照剂量逐渐上升，但在4Gy组其表达不再增高，Bcl-2蛋白在1．0、2．0、4．0Gy组随剂量增大呈下降趋势。以上结果提示重离子束辐照对Tb细胞有抑制作用，Bax／Bcl-2蛋白表达是重离子治癌的机制之一。
英文摘要：
In order to investigate the effect of carbon ion irradiation on apoptosis and Bax/Bcl-2 expression in human tongue carcinoma cells, exponentially growing human tongue carcinoma cells （Tb） cultured in vitro were irradiated with 0, 0.5, 1.0, 2.0 or 4.0 Gy of 12C6＋ ions respectively. Survival rate of irradiated cells at various doses were measured by MTT assay. The nucleus changes of apoptosis and necrosis of cells stained by Hochest/PI were observed through fluorescence microscope. The cell cycle changes were detected by flow cytometry （FCM）. The expressions of Bax and Bcl-2 were detected by Western blot analysis. The results show that the viability of Tb cells decreases gradually with increment of irradiation doses of carbon ions. The proportions of apoptosis cells in the irradiated groups are significantly higher than those in the control group. There is a positive correlation between irradiation doses and retardation strength in G2/M phase at 24 h after irradiation （P〈0.05）. And the expressions of Bax and bcl-2 are significantly up-regulated and down-regulated respectively by 12C6＋ ion irradiation. It can be concluded from above that cell apoptosis induced by heavy ion with high-LET may be mediated through the Bax/Bcl-2 expression pathway.
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