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中文摘要：
建立一种高效液相色谱法快速定性筛查与准确定量检测葡萄酒中人工合成甜味剂纽甜的方法。采用WatersNova-Pak C18反相色谱柱,以离子对试剂缓冲液：乙腈=60：40（V/V）为流动相,在218nm波长处,对样品进行筛查,纽甜出峰时间约在13.4min;疑似阳性样品在梯度洗脱条件下进行定量检测,纽甜出峰时间约在15.9min,检出限为0.37μg/mL,平均回收率在99%～101%之间,相对标准偏差均小于1.0%（n=5）。本方法简便、准确、实用,在批量样品检测时可节省约50%的分析时间。
英文摘要：
A high performance liquid chromatography （HPLC） method for fast qualitative analysis and accurately quantitative determination of artificial sweetener neotame in wine was developed. Neotame was qualitatively analyzed on a Waters Nova-Pak C18 column with an ultraviolet detector at 218 nm using acetonitrile： ion-pair reagent = 40：60 （VIII） as the mobile phase, and the retention time of neotame was approximately 13.4 min. The contents of neotame in the suspected samples were determined under gradient elution condition, and the retention time of neotame was approximately 15.9 min. The detection limit of the developed method was 0.37 μg/mL, and the mean recovery rate for neotame in blank wine samples with spiked standard was 99%-101% with a relative standard deviation less than 1.0% （n = 5）. This method is simple, accurate and practical, and can save 50% time during the analysis of large number of samples.
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