欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的研究多重耐药鲍曼不动杆菌（MDR—ABA）gyrA基因突变与环丙沙星耐药关系。方法以鲍曼不动杆菌影科基因序列为靶序列，用PCR＋DNA测序等方法对62株临床分离株gyrA基因突变进行对比研究。结果在53株耐环丙沙星MDR—ABA菌中，有52株gyrA基因的83位表现出突变，其突变方式全为TCA→TTA，导致氨基酸丝氨酸→亮氨酸；8株环丙沙星敏感和1株环丙沙星中介的MDR—ABA菌gyrA基因无突变。结论临床分离的MDR—ABA菌对环丙沙星耐药的分子机制主要表现为甜科基因83位氨基酸密码子突变。
英文摘要：
Objective To study the relationship between mutation of gyrA gene of multidrug resistant Acinetobacter baumannii （MDR- ABA） and the resistance to eiprofloxacin. Methods With gyrA gene sequence of ABA as target sequence, mutation of gyrA gene of 62 strains of ABA were analyzed by using PCR and DNA sequencing. Results Among 53 strains of ABA which were resistant to eiprofloxacin, 52 strains showed single point TCA→TTA mutation in the gyrA gene at codon 83. Leading to amino acid substitution of Ser→Leu. Eight strains of ABA which were sensitive to ciprofloxacin and 1 strain of ABA which was intermediary to ciprofloxacin showed no mutation. Conclusion The molecular mechanism of the resistant MDR-ABA clinically isolated was the mutation in gyrA gent at eodon 83.
同期刊论文项目
同项目期刊论文
期刊信息
位置：