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中文摘要：
目的建立测定腺梗豨莶中豨莶精醇、16β,17-二羟基-贝壳杉烷-19-羧酸、16α氢-贝壳杉烷-17,19-二羧酸和3＇,4＇-二甲氧基槲皮素含量的高效液相色谱示差折光检测方法（HPLC-RID）。方法以十八烷基硅烷键合相为固定相,流动相为体积分数0.3%甲酸溶液-乙腈（体积比42∶55）,流速为1.0 mL·min-1,示差检测器光学元件温度为40℃,柱温30℃。结果豨莶精醇的线性范围为0.202-2.02 g·L-1,16β,17-二羟基-贝壳杉烷-19-羧酸的线性范围为0.510-5.10 g·L-1,16α氢-贝壳杉烷-17,19-二羧酸的线性范围为0.298-2.98 g·L-1,3＇,4＇-二甲氧基槲皮素的线性范围为0.100-1.00 g·L-1,4种化合物线性关系良好（r〉0.999）;平均加样回收率在99.2%-104.9%内;RSD小于3.4%。结论该方法简便、准确、重复性良好,适用于腺梗豨莶中4种成分的同时测定。
英文摘要：
Objective To establish an HPLC-RID method for simultaneous monitoring of four major constituents in Siegesbeckia pubescens Makino. Methods The separation of four compounds w as achieved w ith a Waters Symmetry ShieldTMRP18 column（ 250 mm × 4. 6 mm,5 μm） and the mobile phase w as 0. 3% formic acid-acetonitrile（ V∶ V = 42∶ 55）. The temperatures of the optical element and column w ere 40 ℃ and 30 ℃,respectively. The flow rate w as 1. 0 mL·min- 1. Results This method w as linear for all the four compounds w ith correlation coefficients 0. 999 0. The calibration curve w as constructed using four calibration samples in the range of 0. 202-2. 02 g·L- 1for darutigenol,0. 510-5. 10 g·L- 1for 16β,17-dihydroxy-kauran-19-oic acid,0. 298-2. 98 g·L- 1for 16α H-kauran-17,19-dioic acid and 0. 100-1. 00 g·L- 1for 3＇,4＇-dimethoxy quercetin. Accuracy w as validated on the basis of quotient of the averaged measurements and the nominal value and it ranged from 99. 2% to 104. 9%. The RSD w ere w ithin 3. 4%. Conclusions The method w hich affords simplicity,high accuracy and excellent precision is successfully applied to determine the four major constituents in Siegesbeckia pubescens Makino simultaneously.
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