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中文摘要：
目的研究紫杉醇分子印迹聚合物在固相萃取中的应用。方法采用分子印迹沉淀合成技术,以紫杉醇为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸为交联剂、偶氮二异丁腈为引发剂,在氯仿与甲苯混合液中合成紫杉醇分子印迹聚合物,并以此为填料进行固相萃取,分离纯化紫杉醇。印迹聚合物对紫杉醇的吸附选择性优化聚合反应条件,通过平衡结合法和Scatchard模型评价聚合物吸附特点,再用固相萃取技术对清洗洗脱方法进行优化,采用实际提取浸膏考察固相萃取柱的可用性。结果甲基丙烯酸和紫杉醇的摩尔比为4∶1,聚合温度为60℃时,分子印迹聚合物（molecularly imprinted polymers,MIPs）对紫杉醇呈现高特异性吸附;印迹吸附符合Scatchard模型,计算MIPs对紫杉醇的最大表观结合点数为3.66 mg·g-1,Kd为6.627。结论分子印迹聚合物可以为紫杉醇的分离纯化提供新型选择性富集材料。
英文摘要：
Objective To investigate the application of solid phase extraction in paclitaxel molecularly imprinted polymer.Methods Molecularly imprinted polymer was prepared through molecular imprinting technique by using paclitaxel as the template molecule,methacrylic acid as the functional monomer,ethylene glycol dimethacrylate as the cross-linker and 2,2＇-azobisisobutyronitrile as the initiator in the porogen of the mixture for chloroform and methylbenzene.The polymer was loaded into the self-made solidphase extraction cartridge.Results The results showed that when the ratio of functional monomer and template molecules is 4∶1,polymerization temperature was 60 ℃,the adsorption effect was well.MIPs in acetonitrile solvent adsorption quantity was moderate,MIPs was 2.68 mg·g-1,NMIPs was 1.21 mg·g-1,however the selectivity was well,MIPs adsorption quantity was significantly higher than NMIPs.Scatchard analysis model of the molecularly imprinted polymer,r2=0.977 9,showed that imprinting adsorption conformed to Scatchard model,the calculated MIPs of paclitaxel Qmax was 3.66 mg·g-1,Kd was 6.627.Conclusions Molecularly imprinted polymer can provide new materials with selectivity and enrichment for the separation and purification of paclitaxel.
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