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中文摘要：
目的研究异去氢钩藤碱在人和大鼠肠道菌群中代谢产物的异同,阐明肠道菌群对异去氢钩藤碱在体内吸收和代谢的影响。方法采用UHPLC/Q-TOF MS技术,鉴定异去氢钩藤碱在人和大鼠肠道菌群培养液中的代谢产物。采用色谱柱为HSS C18（100 mm×2.1 mm,1.8μm）柱,流动相为乙腈-体积分数0.1%甲酸水溶液梯度洗脱,流速为0.5 m L·min-1,采用ESI离子源,正离子模式检测。结果通过与标准品比对保留时间和质谱信息,鉴定了异去氢钩藤碱在人和大鼠肠道菌群培养液中9个代谢产物。其中异构化和还原为异去氢钩藤碱在肠道菌群中的主要代谢途径,水解、羟基化和氮氧化为微量代谢途径。结论建立采用UHPLC/Q-TOF MS方法鉴定异去氢钩藤碱肠道菌群代谢产物,为四环氧化吲哚类生物碱在体内吸收和代谢机制研究提供科学依据。
英文摘要：
Objective To demonstrate the effects of intestinal bacteria on the absorption and metabolismof isocorynoxeine（ IC） in vivo and to compare the difference in the metobolites of IC produced by rat or human intestinal bacteria. Methods An ultra-high-performance liquid chromatography / quadrupole time-of-flight mass spectrometry（ UHPLC / Q-TOF MS） method was developed to identify IC metabolites in human and rat intestinal bacteria. The utilized column was an ACQUITY HSS C18column（ 100 mm × 2. 1 mm,1. 8 μm）,and the mobile phase was composed of 0. 1% formic acid in water（ solvent A） and acetonitrile（ solvent B） in gradient elution and at 0. 5 m L·min- 1flowrate. Analyses were performed using an electro spray ionization（ ESI） source operated in positive ion mode. Results A total of 9 metabolites were identified in the cultured medium of IC in rat and human intestinal bacteria by comparing retention time,characteristic molecular ions and fragment ions with those of the authentic standard. The result indicated that the isomerization and reduction are the major metabolic ways,and that hydrolysis,hydroxylation and N-oxidation are the minors.Conclusions A simple,fast and sensitive method was established for identification of IC metabolites transformed by human and rat intestinal bacteria. Our study is helpful for the further absorption and metabolism study of tetracyclic oxindole alkaloids in vivo.
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