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中文摘要：
目的研究离体氟斑牙早期龋模型经再矿化处理后，釉质表面显微硬度及微观形貌的变化。方法应用离体氟斑牙制备釉质样本40个，经酸蚀凝胶人工龋实验脱矿后，1．0％酪蛋白磷酸肽-磷酸钙（CPP—CP）再矿化液再矿化。采用显微硬度计测定各样本脱矿前后及再矿化后的显微硬度值。采用体视显微镜、偏光显微镜、透射显微镜以及扫描电镜观察釉质表面微观形貌。结果脱矿前，釉质表面显微硬度值为（241．53±21．31）kHV；脱矿后，表面显微硬度下降到（175．76±24．99）kHV（P〈0．05）。其表面硬度损失百分比（％SMHD）为（27．23±4．79）％，表现为类似早期龋的表层下脱矿；再矿化后，表面显微硬度提高到（210．17±21．48）kHV（P〈0．05），其表面显微硬度恢复的百分比（％SMHR）为（52．32±4．23）％，主要表现为表层下再矿化。结论再矿化处理对治疗氟斑牙早期龋有较好的临床效果。
英文摘要：
Objective To investigate the transformation of microhardness and microstructures of human dental fluorosis after demineralization and remineralization in vitro. Methods Forty human dental fluorosis enamel blocks were demineralized by acid-etching gel, then subjected to 1.0% casein phosphopeptides stabilized calcium phosphate （CPP- CP） for remineralization. Surface enamel microhardness was measured on the enamel blocks before and after demineralization and after remineralization. The enamel specimens was observed by stereomicroscope and scanning electron microscope, then sectioned （100 μm） and examined by transmission light microscope and polarized light microscope. Results Before demineralization, the average knoop hardness value of the fluorosed teeth was （241.53±21.31） kHV. After demineralization, obvious decrease of the surface hardness of the enamel was observed, the mean value was （175.76±24.99）kHV（P〈0.05）, the percent of the surface microhardness demineralization（%SMHD） was （27.23± 4.79）%, and major demineralization was under the surface of the enamel, which was similar to the early natural enamel caries. After remineralization, obvious increase of the surface hardness of the enamel was observed, the mean value was （210.17±21.48）kHV （P〈0.05）, the percent of the surface microhardness remineralization （%SMHR） was （52.32±4.23）%, major remineralization was under the surface of the enamel. Conclusion Remineralization could be used to prevent and cure the early natural enamel caries of fluorosed dental teeth.
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