对25份美国甘薯[Ipomoeabatatas(L.)Lam.]品系及另外作为对照的47份甘薯种质资源进行ISSR分析,利用NTSYS软件分析遗传相似系数,采用UPGMA方法进行聚类,构建亲缘关系系统图。结果显示:10个引物共扩增出78个条带,其中有73条呈现多态性,多态位点比例高达93.6%;25份美国甘薯育种材料与对照种质资源的平均遗传相似性系数是0.74。表明美国甘薯育种材料遗传多样性较高,可将其中的6-3—1、6-8—7等品系应用于国内甘薯育种中,以扩大遗传背景。
To understand the genetic diversity of sweetpotato (Ipomoea batatas) collected from USA, 72 sweetpotato breeding lines were analyzed using ISSR markers. The genetic similarity of genetic relationship was calculated by NTSYS software, and dendrogram was clustered by UPGMA method. Based on 73 polymorphic bands amplified from ten primer pairs, genetic similarities among 25 USA lines and 47 control lines ranged from 0. 52 to 0. 94, with an average genetic similarity of 0. 74. The results showed that sufficient polymorphism could be found among USA sweetpotato breeding lines, and some lines, such as 6-3-1and 6-8-7 , could be potential for sweetpotato hybridization breeding.