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Molecular Characterization and SNP Markers of the β-purothionin Gene in Einkorn Wheats
  • ISSN号:1008-4630
  • 期刊名称:《甘肃广播电视大学学报》
  • 时间:0
  • 分类:S512.1[农业科学—作物学]
  • 作者机构:[1]Triticeae Research Institute, Sichuan Agricultural University, Ya "an 625014, P.R. China, [2]Key Laboratory of Crop Genetic Resources and Improvement in Southwest China, Ministry of Education/Sichuan Agricultural University, Ya'an 625014, P.R.China
  • 相关基金:This work was supported by the National Key Technologies R&D Program of China (2006BAD01A02-23 and 2006 BAD13B02-06), and the National High Technology Research and Development Program of China (863 Program, 2006AA10Z179 and 2006AA10Z1F8).
中文摘要:

43 个基因序列编码 purothionin 从单粒小麦小麦的三种或亚种被描绘。这些序列包含了 887 bp,包括 29 indel loci 的 92 SNP 在之中被检测,给每 9.64 个底的一 SNP 的平均 SNP 频率。根据这些序列, 5 个 SNP 标记成功地被设计,它习惯于我的 102 单粒小麦小麦就职的 purothionin 基因的变化。基于 5 检测 SNP loci, 102 单粒小麦小麦就职能被划分成 21 haplotypes, 11 haplotypes 在之中包含了一件单个样品。种系发生的分析显示从单粒小麦小麦的 purothionin 基因比 B 染色体与从 D 染色体的那些有关仔细是更多。七从 43 个基因序列被假定是由包含在里面框架站鳕鱼 ons 和小插入 / 删除导致框架移动的定义的假基因。在留下,在在生物活动起了重要作用的成熟 thionin 领域的 36 个氨基酸序列, 8 Cys 和 Tyr-13 loci 都被保存,而有一些变化发生在象 Lys 和 Arg 那样的一些另外的重要氨基酸残余。

英文摘要:

Forty-three gene sequences encoding purothionin were characterized from the three species or subspecies of einkorn wheats. These sequences contained 887 bp, among which 92 SNPs including 29 indel loci were detected, giving an average SNP frequency of one SNP per 9.64 bases. According to these sequences, 5 SNP markers were successfully designed, which were used to mine the variations ofpurothionin genes of 102 einkorn wheat accessions. Based on the 5 detected SNP loci, 102 einkorn wheat accessions could be divided into 21 haplotypes, among which 11 haplotypes contained a single sample. Phylogenetic analysis indicated that the purothionin genes from einkorn wheats were more closely related to those from D genome than B genome. Seven out of the 43 gene sequences were assumed to be pseudogenes by the definition of containing in-frame stop codons and small insertions/deletions leading to frameshift. In the remaining 36 amino acid sequences, the 8 Cys and Tyr-13 loci in the mature thionin domain which played important roles in the biological activities were all conserved, whereas there were some varieties occurred in some other important amino acid residues such as Lys and Arg.

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期刊信息
  • 《甘肃广播电视大学学报》
  • 主管单位:甘肃省教育厅
  • 主办单位:甘肃广播电视大学
  • 主编:熊进
  • 地址:兰州市南滨河东路571号
  • 邮编:730030
  • 邮箱:yys@gsrtvu.cn
  • 电话:0931-8823542 8721727
  • 国际标准刊号:ISSN:1008-4630
  • 国内统一刊号:ISSN:62-1140/G4
  • 邮发代号:
  • 获奖情况:
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  • 中国国家哲学社会科学学术期刊数据库
  • 被引量:1437