中文摘要：

目的探讨噪声暴露强度和累积噪声暴露量（cumulativenoiseexposure，CNE）对铜锌超氧化物歧化酶基因（SOD1）rs2070424、rs10432782单核苷酸多态性（singlenueleotidepolymorphisms，SNPs）与噪声性听力损失（noiseinducedhearingloss，NIHL）易感性关系的影响。方法利用病例对照研究，通过比较同一噪声暴露强度噪声作业人员的左耳3000Hz频段听阈位移情况，筛选出听阈位移最大的10％个体作为本研究的易感人群组，共201例，听阈位移最小的10％个体作为耐受人群组，共202例，并进行相关的职业卫生调查和问卷调查。噪声暴露强度的检测根据GBZ／T189．8—2007《工作场所物理因素测量一噪声》，抽取易感人群和耐受人群空腹外周静脉血5ml用于常规方法抽提基因组DNA，TaqMan探针法化学荧光等位基因鉴别试验检测SNP。结果在噪声暴露强度85。92dB（A）组内，rs2070424AA基因型与GG基因型相比，NIHL的患病风险较低，0R值为0．37（95％CI：0．17～0．80）。CNE〉82dB（A）组内，rs2070424AA基因型是NIHL的保护因素，与GG基因型相比，OR值为0．25（95％C10．09—0．70）。噪声暴露强度85～92dB（A）组携带rsl0432782GG基因型患NIHL的风险与1Tr基因型相比OR值为3．17（95％C1：1．16—6189）。GT基因型与1TT基因型相比OR值为2．39（95％CI：1．16～4．97）。CNE75～82dB（A）组内，携带GG基因型患NIHL的风险与1TT基因型相比，OR值为2．35（95％CI：0．96～5．72），P=0．06，GG基因型可能是NIHL的危险因素。结论噪声暴露强度和累计噪声暴露量对SOD1rs2070424、rsl0432782SNPs与NIHL易感性关联存在影响。

英文摘要：

Objective To explore the effects of noise exposure level and cumulative noise exposure （CNE） on the relationship between rs2070424 and rs10432782 SNPs in SODI and the susceptibility to noise- induced hearing loss （NIHL）. Methods A case-control study was performed for investigating the effects of en- vironmental risk factors on the susceptibility to NIHL in 201sensitive workers and 202 resistant workers. A questionnaire was utilized to investigate the occupational health and to identify the occupational risk factors. The noise exposure levels were detected according to the Chinese standard Measurement of noise in the work- place （GBZFF 189.8-2007）. The peripheral blood samples （5 ml blood for each sample） were from sensitive workers and resistant workers. Genomic DNA was extracted on the basis of the standard procedures of Takara kit. SNPs were detected using standard procedures of TaqMan probe allele identification method. Results In group exposed to 85-92 dB noise （A）, the risk of NIHL in the subjects with the AA genotype of rs2070424 was lower than that in the subjects with the GG genotype, OR=0.37 （95%CI： 0. 17-0.80）. In group exposed to 〉 82 dB CNE （A）, the AA genotype of rs2070424 is a protective factor of NIHL, as compared with the GG genotype, OR=0.25 （95%C1： 0.09-0.70）. In group exposed to 85-92 dB noise （A）, the risk of NIHL in the subjects with the GG gen0type of rs10432782 was compared with the risk of NIHL in the subjects with the TF genotype, OR= 3.17 （95%CI： 1.16-6.89）. The GT genotype was compared with TF genotype, OR=2.39（95% CI： 1.16-4.97）. In group exposed to 75-82 dB CNE （A）, the risk of NIHL in the subjects with the GG genotype was compared with the risk of NIHL in the subjects with the TT genotype, OR=2.35 （95%CI： 0.96N5.72）, P=O.06. The GG genotype may be a risk factor of NIHJ. Conclusion The noise exposure level and CNE may influence the rela- tionship between rs2070424, rs10432782 SNPs in SODI and noise-induced hearing loss.