利用20条ISSR引物,对7份雀麦属种质进行遗传完整性检测,每一份种质包括库存和圃存两个供试亚群体。提取方法为混合取样。本试验7份种质的供试亚群体之间遗传相似性系数除无芒雀麦3为0.78之外,其余均大于0.80。采用聚类分析除无芒雀麦3和杂色雀麦出现分支外,其余种质亚群体分支都按照同一种质群分别聚类。本试验表明雀麦属种质资源在圃存条件下,经多年保存易发生遗传分化,从而影响其遗传完整性。
The genetic integrity of 7 accessions of Bromus stored in genebank of Inner Mongolia investigated by 20 ISSR primers.Each accession was represented by two sub-populations regenerated in different conservation.The genetic similarity between the two sub-populations of the same accession was above 0.80,except for 0.78 in Wumangquemai 3.Cluster analysis based on the similarity coefficient showed that Wumangquemai 3 and Zasequemai diverged from each other,falling into two branches,and each one cluster was created by two sub-populations of the same accessions.The results showed that the Bromus stored outside had much more genetic divergence.