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中文摘要：
TNFAIP1蛋白是第一个被鉴定受肿瘤坏死因子α诱导的蛋白，TNFAIP1蛋白可能参与DNA复制、合成、细胞凋亡以及人类各种疾病的发生等重要过程，但对该基因确切功能和作用机制研究不多。该文利用实时定量PCR的方法检测该基因在几种常见细胞系的表达情况，发现在COS7以及NIH3T3细胞系中高表达，而在HeLa、HepG2、SW480、PanC1、MCF7等癌细胞系中低表达。由此推测TNFAIP1可能在癌症的发生中起到一定的作用。如果把TNFAIP1基因克隆到真核表达载体pCMV—Myc中，转染到HeLa细胞系中，发现过表达TNFAIP1可促进HeLa细胞的凋亡。
英文摘要：
TNFAIP1 was first identified as a tumor necrosis factor α（TNFα） and interleukin-6 （IL-6） inducible protein. Experiments on human and rat suggested the protein may play roles in the DNA repair, DNA synthesis, cell apoptosis and human diseases. But its real function and mechanism have not been reported. In this paper, the expression of TNFAIP1 were determined in a number of cell lines by Real-time PCR. We found that the expression levels of TNFAIP1 are high in COS7 and NIH3T3 cell lines but low in the cancer cell lines. These suggest that TNFAIP 1may be involved in the process of cancer. The over-expression of human TNFAIP Ican accelerate the apoptosis of HeLa cell.
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