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A genetic linkage map with 178 SSR and 1 901 SNP markers constructed using a RIL population in wheat (Triticum aestivum L.)
  • ISSN号:1000-7091
  • 期刊名称:《华北农学报》
  • 时间:0
  • 分类:R0[医药卫生]
  • 作者机构:[1]State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education~Key Laboratoryof Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement,National Plant Gene Research Centre (Beijing), China Agricultural University, Beijing 100193, P.R. China
  • 相关基金:financially supported by the National Natural Science Foundation of China (91435204, 31271710);the National 863 Program of China (2012AA10A309);the Program of Conservation and Sustainable Utilization of Wild Relatives of Crops by the Ministry of Agriculture of China (201003021)
中文摘要:

The construction of high density genetic linkage map provides a powerful tool to detect and map quantitative trait loci (QTLs) controlling agronomically important traits. In this study, simple sequence repeat (SSR) markers and Illumina 9K iSelect single nucleotide polymorphism (SNP) genechip were employed to construct one genetic linkage map of common wheat (Triticum aestivum L.) using 191 recombinant inbred lines (RILs) derived from cross Yu 8679xJing 411. This map included 1 901 SNP loci and 178 SSR loci, covering 1 659.9 cM and 1 000 marker bins, with an average interval distance of 1.66 cM. A, B and D genomes covered 719.1,703.5 and 237.3 cM, with an average interval distance of 1.66, 1.45 and 2.9 cM, respectively. Notably, the genetic linkage map covered 20 chromosomes, with the exception of chromosome 5D. Bioinformatics analysis revealed that 1 754 (92.27%) of 1 901 mapped SNP loci could be aligned to 1 215 distinct wheat unigenes, among which 1 184 (97.4%) were located on one single chromosome, and the rest 31 (2.6%) were located on 2 to 3 chromosomes. By performing in silico comparison, 214 chromosome deletion bin-mapped expressed sequence tags (ESTs), 1 043 Brachypodium genes and 1 033 rice genes were further added onto the genetic linkage map. This map not only integrated genetic and physical maps, SSR and SNP loci, respectively, but also provided the information of Brachypodium and rice genes corresponding to 1 754 SNP loci. Therefore, it will be a useful tool for comparative genomics analysis, fine mapping of QTL/gene controlling agronomically important traits and marker-assisted selection breeding in wheat.

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期刊信息
  • 《华北农学报》
  • 北大核心期刊(2011版)
  • 主管单位:河北省农林科学院
  • 主办单位:山西省农业科学院 山西省农学会 河南省农业科学院 河南省农学会 天津市农业科学院 天津市农学会等
  • 主编:王海波
  • 地址:石家庄市和平西路598号
  • 邮编:050051
  • 邮箱:hbnxb@163.com
  • 电话:0311-87652166
  • 国际标准刊号:ISSN:1000-7091
  • 国内统一刊号:ISSN:13-1101/S
  • 邮发代号:18-10
  • 获奖情况:
  • 中国自然科学核心期刊,全国综合性农业核心期刊,中国科学引文数据库核心期刊
  • 国内外数据库收录:
  • 日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:32962