从酿酒葡萄梅洛特的葡萄皮、葡萄穗轴及梗中分离出30株葡萄内生菌,经过摇瓶液体发酵后检测发酵液提取物,发现有7株内生真菌具有产白藜芦醇的能力。其中菌株MG1发酵产白藜芦醇的能力稳定、产量高,达97μg/L,其他几株菌株白藜芦醇产量较低且不稳定。对菌株MG1的分类鉴定进行重点研究,结合形态学特征及分子生物检测结果,鉴定其为链格孢属菌。
Thirty endophytic strains were isolated from the skin, cob and stem of the resveratrol-rich grape variety Merlot. Through liquid fermentation and HPLC measurement of resveratrol content in the fermentation broth, 7 capable of producing resveratrol were screened out of the 30 isolates. The strain MG1 showed the strongest stability and the highest yield in the production of resveratrol (97 μg/L), whereas other six isolates had a lower and unstable ability to produce resveratrol. The strain MG1 was identified as Alternaria sp. on the basis of its morphological characteristics and internal transcribed spacer (ITS) gene sequence.