中文摘要：

【目的】研究不同遮阴处理对金莲花生长发育和生理响应的影响，旨在寻求适宜金莲花生长的最佳遮阴度和最佳遮阴时间。【方法】以2年龄金莲花为材料，自5月5日至落叶期进行遮阴处理（0％（cK）、40％、60％和80％），测定金莲花形态指标（株高、叶片数量、叶面积、叶柄长、冠幅、花径、单株开花量、群体花期）和生理指标（丙二醛（MDA）、细胞膜透性、超氧阴离子自由基、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、可溶性蛋白含量和叶绿素含量）。采用透射电镜观察不同遮阴处理下金莲花叶片叶绿体超微结构。【结果】遮阴处理促进金莲花生长发育，其中，40％、60％遮阴处理提高了单株开花量和花径，延长了金莲花群体花期；60％和80％遮阴处理下叶绿体内基粒数、基粒厚度和基粒片层显著增加，淀粉粒数量显著减少；随生长发育期延长，金莲花对遮阴的生理响应不同。遮阴40d时，遮阴对金莲花造成一定弱光胁迫，表现在：随遮阴度增加，叶绿素含量、SOD活性持续下降，MDA含量和细胞膜透性逐渐上升；遮阴处理80d时，遮阴促进金莲花生长，表现在：遮阴处理下细胞膜透性、超氧阴离子自由基显著小于对照，叶绿素含量极显著高于对照。其中，60％和80％遮阴分别极显著和显著提高了可溶性蛋白含量和SOD活性；遮阴120d时，遮阴不利于金莲花生长，遮阴处理显著提高了细胞膜透性，极显著降低了POD活性。【结论】5月至6月中旬金莲花以全光照或40％遮阴为宜，夏季高温季节以40％和60％遮阴为宜，夏末、秋初应及时撤去遮阴棚。

英文摘要：

[Objective] This study investigated the effects of shading treatment on growth, development and physiological response of Trollius chinensis Bunge in order to determine the optimum light intensity and best growth period for shading treatment suitable for T. chinensis growth. [Method] Two-year-old T. chinensis plants were subjected to shading treatments of 0, 40%, 60% and 80% of light intensity of sunshine, respectively, from 5th May to defoliation. Morphological indicators were recorded for plant height, the number of leaves, leaf area, petiole length, crown width, diameter of corolla and the number of flowers per plant. Physiological parameters were measured including the malondialdehyde （MDA） content, relative membrane conductivity, superoxide radical content, superoxide dismutase （SOD） activity, peroxidase （POD） activity, soluble protein content and chlorophyll content. The ultrastructure of leaf chloroplast derived from various shading treatments was observed by transmission electron microscopy. [Resultl The activities of growth and development of T. chinensis were promoted by shading treatments, in which, 40% and 60% shading treatments prolonged the flowering periods, the number of flowers per plant and the diameter of corolla were also increased. The number of grana, grana thickness and the number of grana lamellae were increased significantly by 60% and 80% shadingtreatments, but the number of starch grains in chloroplast was significantly reduced by shading treatments. Physiological characteristics of T. chinensis varied between the duration/growth periods when shading treatments were applied. The plants showed a weak light stress response after shading treatment for 40 days. Following the increase on shading durations the chlorophyll content and SOD activity were decreased while MDA content and relative membrane conductivity were increased. Shading treatment for 80 days boosted the growth of T. chinensis. The relative conductivity and the superoxide radical were significantly lower than the