中文摘要：

利用谷氨酰胺转氨酶（TGase）结合微滤技术从乳清中分离纯化酪蛋白糖巨肽（CGMP）。结合微滤技术考察底物浓度、TGase浓度、pH值、温度和反应时间对分离纯化的影响。通过实验得出最佳酶反应条件为底物浓度8%、TGase酶浓度7U/g、pH6.5、温度39℃、反应时间80min、微滤浓缩循环6次,最后经微滤可得到纯度为70%左右的CGMP。

英文摘要：

The present study aimed to develop a new technology of combined TGase-catalyzed cross-linking and microftltrafion for the separation of CGMP from whey powder. The main technological parameters of TGase-catalyzed cross-linking were studied. Subsequently, the productivity, purity and amino acid composition of CGMP product were analyzed. Results showed that a CGMP product with about 70% purity was obtained through 80 min TGase-catalyzed cross-linking with a substrate concentration of 8% and a TGase dose of 7 U/g at pH 6.5, 39 ℃followed by microfiltration for 6 cycles.