中文摘要：

了解服用益生菌小鼠肠道黏膜和组织中细胞因子的变化情况,对阐明其对小鼠肠道黏膜免疫的影响具有重要的价值。本实验将60只小鼠随机分成4组：日食对照组、生理盐水对照组、瑞士乳杆菌灌喂组和大肠杆菌对照组,连续灌喂15d,采集小鼠小肠利用ELISA法测得各组小鼠肠道黏膜和肠道组织中细胞因子的含量,并作分析。研究结果证实,与对照组相比,瑞士乳杆菌灌胃组小鼠肠黏膜和组织中4种细胞因子明显升高,且存在显著性差异（P〈0.05）：白介素-2IL-2在3d前达到最大值、白介素-4（IL-4）在5d达到最高点、白介素-6（IL-6）在5d达到顶峰、干扰素-γ（IFN-γ）在3d前达到最大值;与对照组相比,瑞士乳杆菌灌胃组小鼠Th1型和Th2型T细胞的不存在显著性差异（P〉0.05）,肠道免疫功能未出现异常,而大肠杆菌灌胃组Th1型和Th2型T细胞平衡被打乱,免疫功能出现异常。

英文摘要：

In order to study the effect ofLactobacillus helveticus on intestinal immunity in mice, cytokines in intestinal mucosa and tissue of mice were analyzed. Totally 60 mice were randomly divided into four groups： normal diet group, normal saline control group, Lactobacillus helveticus group, and E. coli group. All mice except the normal diet control group were orally administered corresponding probiotics for continuously 15 days, and then cytokines in intestine mucosa and tissue of mice were detected using ELISA to analyze immunoregulatory function of Lactobacillus helveticus. Lactobacillus helveticus-adrrfirfistered mice had a significantly higher level of four cytokines when compared to control groups （P 〈 0.05）. The highest level of cytokines was observed at 3 days post-administration for IL-2 and IFN-γ, and at 5 days post-administration for IL-4 and IL-6. Meanwhile, no obvious change in population of Thl and Th2 type T cells in intestinal mucosa and intestinal tissue of Lactobacillus helveticus-admirflstered mice was observed; in contrast, the balance of Thl and Th2 type T cells in E. coli-adrrfinistered mice was disturbed, which revealed that Lactobacillus helveticus had a protective effect on intestinal immune.