中文摘要：

研究用酪蛋白糖巨肽（CGMP）刺激体外培养的小鼠骨髓源树突细胞,观察CGMP对树突细胞成熟的影响,初步探讨CGMP作为功能性食品对免疫的调节作用。实验选用6～8周雄性C57BL/6小鼠,分离培养小鼠骨髓细胞,用重组小鼠白细胞介素-4（rmIL-4）、重组小鼠粒细胞巨噬细胞集落刺激因子（rmGM-CSF）诱导其分化,在培养的第6天,分别加入RPMI1640完全培养基作为空白对照,脂多糖（LPS）为阳性对照和0.1、1、10μg/mL的CGMP 3个实验剂量。培养至第8天收集细胞,进行三色荧光标记,用流式细胞仪检测细胞表面标志：CD11c、CD80、CD86、MHC-Ⅱ。另一方面将第8天的树突状细胞（DCs）和T淋巴细胞共孵育进行混合淋巴细胞反应,96h后用CellCounting Kit-8（CCK-8）检测T淋巴细胞的增殖。结果表明：3个剂量的CGMP均具有刺激DCs成熟的作用,其中CGMP为1μg/mL时,DCs的各种成熟标志均达到最高水平,其中,CGMP对MHC-Ⅱ的刺激作用最为强烈,而MHC-Ⅱ在DCs对外源抗原的提呈中发挥重要作用;混合淋巴细胞反应结果显示各个不同浓度CGMP作用的DCs均具有不同程度的刺激T淋巴细胞增殖的能力,并且DCs：T为1：10时T淋巴细胞的增殖作用最明显。

英文摘要：

The present study was undertaken to discover the effect of casein glycomacropeptide（CGMP） on maturation of in vitro cultured mouse bone marrow-derived dendritic cells and gain a preliminary understanding of the immunoregulatory functions of CGMP as a functional food ingredient.Bone marrow-derived dendritic cells（BM-DCs） from 6-8 week-old male C57BL/6 mice were isolated,cultured and differentiated in the presence of both rmIL-4 and rmGM-CSF.On day 6 of the culture process,three doses of CGMP（0,0.1,1.0μg/mL and 10μg/mL）,RPMI 1640 complete medium（blank control） and LPS（positive blank） were added respectively and the BM-DCs were cultured for another 2 days,harvested,labeled with three fluorescence and examined for the surface markers CD11c,CD80,CD86,and MHC-Ⅱ by flow cytometry.Meanwhile,the harvested BM-DCs and T lymphocytes were co-cultured for 96 h before Cell Counting Kit-8（CCK-8） examination of T lymphocyte proliferation resulting from mixed lymphocyte reaction（MLP）.The results showed that all three doses of CGMP could promote the maturation of BM-DCs.All the maturation markers of dendritic cells reached their maximum at the dose of 1 μg/mL.Among the markers,MHC-Ⅱ,which plays a significant role in antigen presentation by BM-DCs,was promoted most.Co-culture of BM-DCs and T lymphocytes showed that BM-DCs treated with CGMP promoted the proliferation of T lymphocytes.T lymphocytes BM-DCs showed maximum proliferation when the initial ratio of BM-DCs to T lymphocytes was 1：10.