中文摘要：

目的观察参芍胶囊预处理对心肌缺血再灌注损伤（MIRI）大鼠心脏组织和功能及过氧化损伤、抗氧化能力的影响。方法采用左冠状动脉前降支结扎30 min,再灌注120 min制备心肌缺血再灌注损伤模型。将30只雄性Wistar大鼠随机分为5组：假手术组、模型组、参芍胶囊250 mg/kg组、参芍胶囊500 mg/kg组和阿托伐他汀组,每组6只。术前用药7 d。再灌注后生理记录仪记录血流动力学变化,生化法检测血清肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）及心肌组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）含量。结果与模型组比较,参芍胶囊高、低剂量组与阿托伐他汀组CK-MB和LDH均明显降低（P〈0.05或P〈0.01）。与参芍胶囊250 mg/kg组比较,参芍胶囊500 mg/kg组和阿托伐他汀组LDH显著降低（P〈0.01）。与模型组比较,参芍胶囊250 mg/kg组左心室内压最大上升速率（＋dp/dt_（max））值明显升高（P〈0.05）;参芍胶囊500 mg/kg组和阿托伐他汀组LVSP和-dp/dt_（min）值明显升高（P〈0.05）,＋dp/d_（max）值显著升高（P〈0.01）,左心室舒张末压（LVEDP）值明显降低（P〈0.05）。与模型组比较,参芍胶囊高低剂量组与阿托伐他汀组心肌中丙二醛（MDA）明显降低（P〈0.05或P〈0.01）,超氧化物气化酶、谷胱甘肽过氧化物酶明显升高（P〈0.05或P〈0.01）。结论参芍胶囊预处理能够抑制MIRI后大鼠心肌损伤,改善心脏血流动力学状态,抑制膜脂过氧化产物的生成及提高机体抗氧化损伤能力。

英文摘要：

Objective To observe the effect of Shenshao capsule（ SSC） preconditioning on myocardial ischemia reperfusion injury（ MIRI） in rat heart and the mechanism of oxidative damage and antioxidant capacity. Methods In this study,besides sham group,the left anterior descending coronary artery of other wistar rats were occluded 30 min and reperfused 120 min in MIRI model. Thirty rats were randomly divided into sham group,ischemia / reperfusion group,low and high dose SSC group（250 mg / kg and 500 mg / kg）,and atorvastatin 10 mg / kg group,pre-treated for 7 days. After the end of the reperfusion,physiological recorder was used to measure the cardiac hemodynamic changes. The contents of serum creatine kinase isoenzyme MB（ CK-MB） and lactate dehydrogenase（ LDH） and the myocardial tissue malondialdehyde（ MDA）,superoxide dismutase（SOD）,glutathione peroxidase（GSH-PX） were detected by automatic biochemical analyzer. Results Compared with model group,the contents of serum CK-MB and LDH were significantly lower in low and high dose SSC group and atorvastatin group（P〈0. 05 or P〈0. 01）. Compared with low dose SSC group,the content of LDH was significantly lower in high dose SSC group and atorvastatin group（P〈0. 01）. Compared with model group,＋ dp / dtmaxvalue increased significantly in low dose SSC group（P〈0. 05）. LVSP and-dp /dtmin value increased（P〈0. 05）, ＋ dp / dtmaxvalue increased significantly（P〈0. 01）,LVEDP value decreased（P〈0. 05） in high dose SSC group and atorvastatin group. Compared with model group,the contents of myocardial MDA significantly lower,the contents of SOD and GSH-PX increased significantly in low and high dose SSC group and atorvastatin group（P〈0. 05 or P〈0. 01）. Conclusion SSC pretreatment can inhibit myocardial injury in rats after MIRI,improve cardiac hemodynamic state,inhibit the generation of membrane lipid peroxidation products and improve the body＇s ability to resist oxidative damage.