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中文摘要：
目的：模拟阻塞性睡眠呼吸暂停综合征（OSAS）大鼠模型,研究OSAS继发高血压血管重构的病理形态改变。方法：1将32只SD雄性大鼠按随机列表法分为慢性间歇低氧组（CIH组）与常氧对照组（NC组）,CIH组实验过程中置于低压氧舱中行间歇低氧模式,NC组置于同样规格氧舱中常氧对照。每两周监测尾动脉血压一次,于低氧时程6、12周末对腹主动脉进行HE染色、Masson染色观察其病理形态学改变,采用免疫组织化学检测腹主动脉α-平滑肌肌动蛋白（α-SMA）、增殖细胞核抗原（PCNA）及纤维粘连蛋白（FN）的表达分析腹主动脉增殖及纤维化,PCR检测ECM标志物转化生长因子-β1（TGF-β1）mRNA。结果：与NC组比较,CIH组血压逐渐升高（P〈0.01）;腹主动脉管腔存在重构相关的病理形态学变化,PCNA、FN及TGF-β1mRNA表达均增加,α-SMA表达降低（均P〈0.05）。结论：OSAS大鼠模型存在血压升高及腹主动脉血管重构的病理变化,这将为今后研究OSAS继发高血压的病理生理机制提供更坚实的理论基础。
英文摘要：
Objective： To investigate the pathological changes of vascular remodeling caused by secondary hypertension in OSAS using OSAS rats model. Method： Thirty-two Sprague-Dawley male rats were divided into two groups randomly： the chronic intermittent hypoxia group （CIH group） ,subjected to intermittent hypoxiatthe normoxical control group（NC group）,without hypoxia. Artery pressure was monitored every two weeks. Subjects were sacrificed at 6 th week and 12 th week,and then HE staining and Masson staining were used to explore the morphological changes of abdominal arteries. The expression of a-smooth muscle actin （α-SMA） , proliferating cell nuclear antigen（PCNA） and fibronectin （FN） were observed with immunohistochemical staining to evaluate the proliferation and fibrosis. ECM marker, TGF-βmRNA was detected with PCR. Result：Compared with NC group,artery pressure increased gradually（P〈0.01） ; Vascular remodeling obviously existed in abdominal artery. The expression of PCNA,FN and TGF-β1mRNA rises, while the expression of α-SMA decreased significantly in CIH group （all P〈0.05）. Conclusion： Long-time CIH state could result in increased pressure and abdominal artery remodeling, which may contribute to providing more solid theoretical basis for further research of OSAS combined with hypertension patients.
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