中文摘要：

目的：观察清热化瘀方联合缺氧预处理（HP）骨髓间充质干细胞（BMSCs）移植对脑缺血损伤大鼠血管新生标志物CD31表达变化及神经功能的影响。方法：将120只大鼠随机为6组：假手术组（SO组）、模型组（MCAO组）、BMSCs移植对照组（BMSCs组）、HP-BMSCs移植组（HP-BMSCs组）、BMSCs移植联合清热化瘀方组（中药对照组）以及HP-BMSCs移植联合清热化瘀方组（中药干预组）,各组根据取材时间点又分为7天、14天两个亚组,每个亚组10只大鼠。线栓法建立大鼠大脑中动脉梗阻模型,根据分组情况给予清热化瘀颗粒灌胃及经尾静脉移植BMSCs或HP-BMSCs,参照m NSS评分法进行神经功能评分,采用免疫组化染色法检测血小板-内皮细胞黏附分子（CD31）及Brdu标记的BMSCs细胞。结果：与MCAO组比较,中药对照组、中药干预组、HP-BMSCs组、BMSCs组神经功能评分显著减少,差异均有统计学意义（P〈0.05,P〈0.01）。与SO组比较,其它各组CD31表达均显著上调,中药干预组上调的最为明显,差异均有统计学意义（P〈0.01）。与HP-BMSCs组比较,BMSCs组神经功能评分增加,CD31表达下降,Brdu标记的阳性细胞数较低;中药干预组神经功能评分显著减少,CD31表达明显上调,Brdu标记的阳性细胞数显著增多,差异均有统计学意义（P〈0.05,P〈0.01）。与中药对照组比较,中药干预组神经功能评分明显减少,CD31表达量显著增加,Brdu标记的阳性细胞数显著增多,差异均有统计学意义（P〈0.05,P〈0.01）。结论：清热化瘀方联合HP-BMSCs移植可改善脑缺血损伤SD大鼠的神经功能,可能和其促进大鼠脑缺血区血管新生标志物CD31的表达相关。

英文摘要：

Objective：To observe the effect of Qingre Huayu prescription combined with hypoxic preconditioning（HP） bone mesenchymal stem cells（BMSCs） transplantation on expression of angiogenesis marker CD31 and neurological function of rats with cerebral ischemic injury.Methods：Divided 120 rats into 6 groups randomly：the sham operation group（SO group）,the model group（MCAO group）,the BMSCs transplantation control group（BMSCs group）,the HP-BMSCs transplantation group（HP-BMSCs group）,the BMSCs transplantation combined with Qingre Huayu prescription group（Chine s e medicine control group） and the HP-BMSCs transplantation combined with Qingre Huayu prescription group（Chinese medicine intervention group）.Each group was divided into 7-day and 14-day two subgroups according to the sampling time,10 rats in each subgroup.The middle cerebral artery occlusion rat model was established by the method of suture-occluded method.Qingre Huayu particles were administrated to different groups by gavage and BMSCs or HP-BMSCs was transplanted by tail vein.Scored the neurological function referring to modify Neurological severity scores（mNSS） scoring method.Determined platelet endothelial cell adhesion molecule（CD31） and BMSCs cells marked with Brdu by immunohistochemistry staining method.Results：Scores of mNSS of Chinese medicine control group,Chinese medicine intervention group,BMSCs group and HP-BMSCs group were declined obviously,comparing with that of MCAO group,differences being significant（P〈0.05,P〈0.01）.Compared with SO group,other groups＇ expression of CD31 were all increased significantly,and the most obvious increase was in Chinese medicine intervention group,showing significant differences（P〈0.01）.In comparison of HP-BMSCs group,mNSS of BMSCs group was raised,while expression of CD31 and the amount of positive cells marked with Bru were decreased;mNSS of of Chinese medicine intervention group was declined obviously,expression of CD31 was increased significantly and the am