为深入了解明光小耳猪群体遗传结构,使之得到更好的保护和利用,采用分布在家猪19对染色体上的76个微卫星标记对该猪种65个样品进行群体遗传变异分析。共检测到343个等位基因,每个座位的等位基因数从2到9不等,有效等位基因数在1.2239~4.8079之间,平均每个座位等位基因数为(4.5132±1.2055)个,有效等位基因数为(3.2169±0.7736)个,群体平均表观杂合度、期望杂合度及平均多态信息含量分别为0.9442±0.1595、0.6685±0.0945和0.6103±0.1083。结果表明,明光小耳猪群体遗传变异丰富。
In order to reveal the genetic variation in Mingguang small-ear pig population and provide the genetic background for its preservation and utilization, 65 individuals sampled from the breed were assayed by using 76 microsatellite loci located on swine's 19 pairs of chromosomes. A total of 343 alleles were detected, the number of alleles varied from 2 to 9 and the number of effective alleles from 1. 223 9 to 4. 807 9, giving mean allele number at each locus was 4. 513 2 ± 1. 205 5 and effective allele number was 3. 216 9±0. 773 6. The mean observed heterozygosity, expected heterozygosity and polymorphism information content (PIC) in the population were 0. 944 2±0. 159 5, 0. 668 5±0. 094 5 and 0. 610 3±0. 108 3, respectively. The results indicated that the genetic variation was rich in this population.