中文摘要：

亲子鉴定在畜牧业生产中具有重要实际应用价值，常见其用于建立准确的系谱档案和家畜归属的诉讼案件。其中标准的三联体（即在母子关系是已知的情况下，鉴定假设父亲与子畜是否有亲生关系）和单亲[即父子（女）或母子（女）亲生关系的确定]两种情况下的亲子鉴定在畜牧生产中具有普遍意义。以云南文山黄牛为试验材料，采用10对常染色体微卫星DNA标记对这两种情况下的亲子关系进行了检测分析。结果表明，常染色体微卫星DNA标记在标准的三联体和单亲两种情况下都能用来进行亲子鉴定，结果较准确，但标准三联体情况下的亲子鉴定更准确。确定的不同模式下亲子鉴定的方法，可为家养动物亲子鉴定标准方法和流程的建立提供依据。

英文摘要：

Parentage identification is of the important application in animal husbandry. It is normally applied in establishing accurate pedigree archives and attributive lawsuit for livestock. Standard triplet （viz. female parent, her offspring and alleged father parent） and single parent （viz. offspring and alleged female or male parent） testings are the familiar cases in the parentage identification of domestic animals. In this study, standard triplet and single parent testing cases in Wenshan cattle （Bos taurus） were carried out by using microsatellite DNA markers. The results indicated that the autosomal microsatellite DNA marker can be used to examine the parenthood in the two cases, but the triplet testing was more precise than single parent testing. The protocol and methods established in this study can be used as the reference for the parentage identification and analysis in other domestic animals in the future.