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辣椒制品金黄色葡萄球菌污染及肠毒素污染研究
  • ISSN号:1000-9973
  • 期刊名称:《中国调味品》
  • 时间:0
  • 分类:TS207.4[轻工技术与工程—食品科学;轻工技术与工程—食品科学与工程]
  • 作者机构:[1]贵州出入境检验检疫局检验检疫综合技术中心,贵阳550004
  • 相关基金:贵州省高层次人才科研条件特助经费项目(TZJF-2007年-18号);国家质检总局科技计划项目(2009IK165)
中文摘要:

了解辣椒制品金黄色葡萄球菌的污染状况,检测辣椒制品中金黄色葡萄球菌携带肠毒素的情况,为开展辣椒制品安全风险评估,企业及产品分类管理提供必要的依据。方法:参照GB4789.10—2010对样品进行金黄色葡萄球菌分离、培养,用VITEK 2 compact全自动微生物鉴定分析系统进行生化鉴定,同时用全自动荧光酶标免疫测试系统(VIDAS30)检测分离菌株携带肠毒素SEA-SEE情况。结果:从705份样品中共检出金黄色葡萄球菌21株,总检出率为2.98%。其中干辣椒制品未检出金黄色葡萄球菌、油辣椒检出率为3.34%,发酵辣椒制品检出率为5.63%,其他辣椒制品检出率为1.32%。21株金黄色葡萄球菌中11株葡萄球菌肠毒素阳性,总检出率为52.38%。其中油辣椒葡萄球菌肠毒素阳性率为46.67%,发酵辣椒制品葡萄球菌肠毒素阳性率为75.00%,其他辣椒制品葡萄球菌肠毒素阳性率为50.00%。辣椒制品样品产地涉及15个省(区、市),从4个省(区)产的辣椒样品中检出金黄色葡萄球菌并携带肠毒素。结论:不同类型辣椒制品污染程度存在差异,不同产地辣椒制品污染程度存在差异,辣椒制品存在金黄色葡萄球菌污染风险,并且产肠毒素的金黄色葡萄球菌占一定的比例。

英文摘要:

Objective. To realize the contamination situation of Staphylococcus aureus in chili condiment, and research the distribution of Staphylococcal enterotoxins of strains aimed for product Security risk assessment,corporate and product category management. Methods.. The methods was developed according to GB 4789.10-2010, with the VITEK 2 compact automated microbial identification system for biochemical analysis, enterotoxins SEA-SEE situation of strains with Automatic fluorescence enzyme immunoassay test system(VIDAS 30)method was detected. Results. 21strains of Staphylococcus aureus were detected from 705 samples,the detectable rate of staphylococcus aureus is 2.98%, Dried chili products which were not detected in Staphylococcus aureus,the detection rate of oil Chili products was 3.34% ,chili fermentation products was detected in 5.63%, other chili products was detected in 1.32%. Among 21strains of Staphylococcus aureus, 11 strains of Staphylococcus enterotoxins was positive , the total detection rate was 52.38%. Staphylococcal enterotoxin of oil Chili products was 46.67% positive, chili fermentation products was 75.00%, other chili products was 50.00%. Chilli condiment samples involved 15 provinces (autonomous regions and municipalities) , Staphylococcus aureus and Staphylococcal enterotoxins was found from four provinces (regions) in the chili condiment samples. Conclusion: The contamination level of different types of chili condiment are different, the contamination level of chili condiment from different areas are different. There is risk of contamination of Staphylococcus aureus of chili condiments, Staphylococcal enterotoxins was certain proportion of strains of Staphylococcus aureus detected in chili condiments.

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期刊信息
  • 《中国调味品》
  • 北大核心期刊(2011版)
  • 主管单位:中国商业联合会
  • 主办单位:全国调味品科技情报中心站
  • 主编:杨旭
  • 地址:哈尔滨市利民经济开发区南京路6号
  • 邮编:150025
  • 邮箱:zgtwp1976@163.com
  • 电话:0451-87137077,87137088
  • 国际标准刊号:ISSN:1000-9973
  • 国内统一刊号:ISSN:23-1299/TS
  • 邮发代号:14-13
  • 获奖情况:
  • 商业部科技进步奖,国内贸易部评优秀期刊,RCCSE中国核心学术期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:13476