红树属植物 endophytic 真菌 1893 在华南海海岸上从河口红树属植物从 Kandelia candel 被孤立。和另外的已知的混合物,二新内酯 1893A 和 1893B 从它的发酵肉汤被孤立。为进一步工业的申请正确地分类内部寄生植物,词法、分子的技术的联合被用来接近它的身份。内部寄生植物与有显然与从包围包围的 ascogonial 卷发展的象雄器一样 hyphae,和 perithecia initials 熔化了的 trichogynes 或象 trichogyne 一样 hyphae 的类似的种类相比在纯粹培养上的早发育阶段的 hyphae。当内部寄生植物在 Kandelia candel 和 Mangifera indica 的叶子被栽培时,宿主和非主人上的进一步词法的特征与类似的种类被用于比较,分别地它导致了作为 Phomopsis 种分类内部寄生植物。它 rDNA 的顺序被用来与在形态学或生态学类似于种类的 Phomopsis 种类推断它的种系发生的关系。最后,内部寄生植物作为 Diaporthe phaseolorum var 被识别。sojae 基于词法、分子的证据。我们的学习是 Diaporthe phaseolorum var 的第一份报告。从红树属植物 Kandelia candel 孤立的 sojae。
Mangrove endophytic fungus 1893 was isolated from Kandelia candel from an estuarine mangrove on the South China Sea Coast Two new lactones 1893A and 1893B, together with other known compounds, have been isolated from its fermentation broth. To classify the endophyte correctly for further industrial application, a combination of morphological and molecular techniques was used to approach its identity. The endophyte was compared with similar species having trichogynes or trichogyne-like hyphae which apparently fused with an- theridium-like hyphae, and perithecia initials developing from an ascogonial coil surrounded by enveloping hyphae in early developmental stages on pure culture. Further morphological characteristics on host and non-host were used for comparison with similar species when the endophyte was cultivated on leaves ofKandelia candel and Mangifera indica, respectively, which resulted in classifying the endophyte as a Phomopsis specics. The ITS sequence of rDNA was used to infer its phylogenetic relationships with Phomopsis species that resembled the strain in morphology or ecology. Finally, the endophyte was identified as Diaporthe phaseolorum var. sojae based on morphological and molecular evidence. Our study is a first report ofDiaporthephaseolorum var. sojae isolated from mangrove Kandelia candel.