中文摘要：

利用SAS软件的Plackett-Burman设计法,对影响南海红树林真菌1403的菌体生长和灰黄霉素生产的培养基进行筛选,所选取的8个相关因素为：pH值、葡萄糖浓度、蛋白胨浓度、酵母膏浓度、MgCl2.6H2O浓度、NaCl浓度、K2HPO4浓度、ZnSO4浓度。结果表明：对菌体生长最显著影响的因素是：葡萄糖浓度（P=0.001 3）和MgCl2.6H2O浓度（P=0.001 5）。对培养液中灰黄霉素的代谢量最显著影响的因素是：葡萄糖浓度（P=0.003 0）,NaCl浓度（P=0.002 6）,MgCl2.6H2O浓度（P=0.011 0）,K2HPO4浓度（P=0.010 7）,ZnSO4浓度（P=0.012 1）。此筛选结果为工业发酵提供了条件。

英文摘要：

Two level factorials designs of Plackett-Burman were constructed to select culture medium components of the mycelial growth and griseofulvin in broth. Firstly, Plackett-Burman design was employed to evaluate the effects of the eight components in the medium, including pH, the contents of glucose, peptone, yeast extract, MgCl2·6H2O, NaCl, KEHPO4 and ZnSO4. It was confirmed that two important components which most affected the mycelial growth were glucose （P = 0. 0013） and MgCl2·6H2O （P = 0. 0015）. Meanwhile, glucose （P = 0.0013）, NaCl（P = 0.0026） , MgCl2·6H2O （P = 0.0110） , KEHPO4 （P = 0.0107） and ZnSO4 （P = 0. 0121 ） determined the metabolism of Griseofulvin in the broth. The result is useful for further study of the industrial fermentation.