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二氧化硫暴露对拟南芥叶片形态和生理生化指标的影响
  • ISSN号:1672-2043
  • 期刊名称:《农业环境科学学报》
  • 时间:0
  • 分类:X503.233[环境科学与工程—环境工程]
  • 作者机构:[1]山西大学生命科学与技术学院,山西太原030006
  • 相关基金:国家自然科学基金(30470318);山西省留学回国基金项目
中文摘要:

采用室内培养及密闭箱静态熏气方法,研究了不同浓度SO2暴露对拟南芥(Arabidopsis thaliana)叶片形态和生理生化指标的影响。结果显示,拟南芥暴露于SO214d后,叶面气孔开度变小。浓度10mg·m^-3组促进植株生长发育,SOD和CAT活性无明显改变,30mg·m^-3SO2组3种酶活性呈诱导性增高,其中POD增幅最大,SO2浓度90mg·m^-3时,CAT活性被抑制,丙二醛(MDA)含量增加,株高、叶片数和叶片面积显著降低,可溶性蛋白含量减少,叶片出现明显可见伤害。研究结果表明,在低浓度条件下,拟南芥能够通过调节气孔开放、细胞抗氧化酶活性等使植株适应SO2胁迫,但是高浓度SO2暴露会引起细胞氧化损伤,影响植物生长发育。

英文摘要:

Effects of SO2 exposure on the morphological and physiological biochemical parameters were investigated in model plant A rabidopsis thaliana. Test plants were exposed to SO2 at concentrations of 10, 30 and 90 mg·m^-3, respectively, 4 h per day for 14 d in an environment-controlled chamber, and then some parameters were measured by detecting the stomatal aperture, activities of antioxidant enzymes and growth rate. The results showed that stomatal aperture and the ratio of stomatal opening area decreased with an increase of the amount of closed stomata when plants exposed to SO2. The activities of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) increased dose-de-pendently in Arabidopsis thaliana plants. 10 mg·m^-3 SO2 exposure had no markedly effect on the activities of SOD and CAT, and showed a positive effect on plants growth and development. 30 mg·m^-3 SO2 exposure significantly enhanced the activities of the three antioxidant enzymes, and POD activity had the maximum increment. Exposure to 90 mg·m^-3 SO2 caused decreases in the activity of CAT and the content of soluble proteins, and inhibition of plant growth and development, but an increase in the content of malondialdehyde (MDA). Our results suggested that A rabidopsis thaliana can adapt to SO2stress by controlling stomatal opening and activities of antioxidant enzymes, however, exposure to higher concentration can cause oxidative damage to plants.

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期刊信息
  • 《农业环境科学学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国农业部
  • 主办单位:农业部环境保护科研监测所 中国农业生态环境保护协会
  • 主编:李文华
  • 地址:天津市南开区复康路31号
  • 邮编:300191
  • 邮箱:caep@vip.163.com
  • 电话:022-23674336 23006209
  • 国际标准刊号:ISSN:1672-2043
  • 国内统一刊号:ISSN:12-1347/S
  • 邮发代号:6-64
  • 获奖情况:
  • 全国中文核心期刊,列于被引频比最高的中国科技期刊500名之内
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:34877