运用拟南芥寡核苷酸芯片ATH1,分析SO2胁迫对拟南芥基因表达谱的影响.在22 810个探针中共检出30 mg.m-3SO2胁迫组与对照组间表达改变1倍以上的基因494个,其中上调220个,下调表达274个,主要涉及与细胞代谢(189个)、结合(177个)、转录调控(74个)、结构分子(55个)、信号转导(53个)、物质运输(39个)等功能相关的基因.胁迫组中与细胞防御相关的基因,包括防御酶基因、病程相关蛋白PRs和细胞壁防御相关基因等表达上调.结果表明,SO2胁迫时植物细胞能够通过对基因转录的调控,从分子水平上改变细胞的生理过程,提高植株对逆境的适应性.
A genome-wide microarray with 22 810 25-mer oligonucleotides covering the presently known and predicted genes in Arabidopsis thaliana genome was used to survey the gene expression in aerial part of Arabidopsis thaliana on day 3 of SO2-fumigation. There were 494 genes expressed differently in 30 mg · m^-3 SO2-fumigation A. thaliana shoots,they reflected well major stress-specific functions on the molecular level. Defense genes expression was induced to enhance the capacity of plants to resistance to SO2. The data presented in this study provide a new resource for annotating A. thaliana genome.