以大蒜为材料,研究不同酸度(pH4.5和pH5.8)的铝溶液对蒜苗根系的氧化损伤和遗传损伤.结果表明,经10umol/L~1000umol/L的铝处理后,大蒜根系抗氧化酶SOD、POD、CAT活性改变,pH4.5的铝组酶活性呈诱导性增高,而pH5.8时高浓度组酶活与对照接近,其余铝组酶活性降低,但pH4.5和pH5.8的铝处理均能使细胞MDA含量增加.5umol/L~100umol/L的铝处理能导致大蒜根尖微核细胞率增加,且显著增加的铝浓度在pH4.5时较低.研究结果表明,铝对植物细胞具有遗传损伤作用,可能与铝胁迫引起的氧化损伤有关,环境酸度增强能加重铝对植物的毒害.
Genotoxicity and oxidative damage of aluminum (A1) on plant cells was studied in Allium sativum L. root cells at pH 5. 8 and pH 4. 5, respectively. Exposure to 10 umol/L to 1 000 umol/L aluminum trichloride solution containing 100 umol/L Ca^2+, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) increased significantly in plant roots at pH 4. 5, but enzymes activities decreased at pH 5. 8 except in higher concentration treatment groups. However, A1 treatments both at pH 4. 5 and pH 5. 8 caused marked increases in MDA contents in root cells. Furthermore,Al treatments induced micronucleus formation in the range of 5 to 100umol/L,and higher toxicity occurred in pH 4.5 groups than those in pH 5.8 groups. Our results indicate that aluminum trichloride is a genotoxic agent in plant cells,and its toxicity is enhanced at low pH value. Our results suggest that the genoxicity may result from the oxidative stress by Al.