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中文摘要：
以拟南芥叶片下表皮为材料，研究了SO2体内衍生物——亚硫酸钠和亚硫酸氢钠混合液（3：1，mmol·L^-1／mmol·L^-1）对气孔保卫细胞的致死作用。结果表明，浓度0．5～4．5mmol·L^-1的SO2衍生物处理表皮3h可引起保卫细胞死亡，细胞死亡率呈浓度依赖性增高。抗坏血酸（AsA）或过氧化氢酶（CAT）与SO2衍生物共同作用时，保卫细胞死亡率显著降低。Ca^2＋合剂乙二醇双四乙酸（EGTA）或Ca^2＋通道抑制剂LaCl3与SO2衍生物共同作用时，保卫细胞死亡率亦显著降低。研究发现，一定浓度的SO2可诱导拟南芥保卫细胞死亡，胁迫可能通过诱导活性氧、激活质膜钙通道，造成胞外Ca^2＋内流，引发细胞死亡。
英文摘要：
The effects of SO2 derivatives on guard cells death were investigated in model plant Arabidopsis thaliana. The isolated epidermal strips were treated with various chemicals under white light for 3 h, stained with FDA, and examined with a fluorescence microscope. Randomly selected views on the tested epidermal strips were monitored to determine the number of viable cells and the total number of scored cells. SO2 derivatives, a mixture of sodium sulfite and sodium hisulfite （3：1, mmol·L^-1/mmol·L^-1）, significantly induced cell death at con- centrations from 0.5 to 4.5 mmol·L^-1. However, the percentage of the cell death decreased when the strips exposed to SO2 derivatives combined with ascorbic acid （AsA, 0.1, 1.0 mmol·L^-1） or catalase （CAT, 200 U·mL^-1）. The cell death percentage also decreased after the strips exposure to SO2 derivatives combined with EGTA （Ca^2＋ chelator, 0.1, 1.0 mmol·L^-1） or LaCl3 （Ca^2＋ channel blocker, 0.1mmol·L^-1）. The results of the present study indicated that SO2 caused cell death in A. thaliana via reactive oxygen species （ROS） production. ROS activated plasma membrane Ca^2＋ channels, led to Ca^2＋ influx and an increase of intracelluar Ca^2＋ level, and induced finally cell death.
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