中文摘要：

目的探讨WT1基因启动子甲基化与新疆维吾尔族妇女宫颈癌的相关性。方法采用PCR．方法检测HPV16和HPV18感染率；甲基化特异性PCR方法检测43例新疆维吾尔族妇女正常宫颈组织、30例宫颈上皮内瘤样病变（CIN）和48例宫颈癌组织的WT1基因启动子两个区域甲基化状况，并分析启动子甲基化与宫颈癌的相关性。结果正常宫颈组、CIN和宫颈癌组中的WT1基因甲基化率分别为6．98％、36．67％和89．58％；HPV16和HPV18的感染率分别为13．95％、33．33％和68．75％，3组差异均有统计学意义（P〈0．01）。WT1甲基化与HPV16和HPV18感染相关（P〈0．05）。结论WT1基因启动子甲基化与HPV16和HPV18感染相关且与维吾尔族宫颈癌发生相关。

英文摘要：

[ Objective ] To explore the correlation between WT1 gene promoter methylation and cervical cancer of Uyghur women. [ Methods ] Forty-three cases of normal cervical tissues, 30 cervical intraepithelial neoplasia （CIN） and 48 cases of cervical cancer tissues were obtained by surgery from Uyghur women of Xinjiang. HPV16/18 infection was detected by PCR method. Methylation-specific PCR method was used to analyze the methylation in two regions of WT1 gene promoter. Then the correlation between promoter methylation and cervical cancer was analyzed.[Results] The methylation frequency of WTl gene promoter from normal cervical tissues to cervical cancer tissues was 6.98%, 36.67% and 89.58%, respectively; the infection frequency of HPV16/18 was 13.95%, 33.33% and 68,75%, respectively, and the difference was statistically significant （P〈0.01）. The correlation between methylation ratio of WT1 and infection ratio of HPV16/18 was statistically significant （P〈0.05）. [Conclusions] WT1 gene promoter methylation is correlated with the infection of HPV16/18 and the cervical cancer of Uyghur women.